1,5-naphthylenediamine

Endpoint summary

Additional information

1,5-Naphthylenediamine showed mutagenic properties in bacteria with (S. typhimurium TA 1537, TA 1538, TA 98 and TA 100) and without metabolic activation (S. typhimurium TA 1537, TA 98 and TA 100; Bacillus subtilis H17). A gene mutation assay with Chinese hamster V79 cells and two in vitro UDS tests with primary rat hepatocytes gave no indications for mutagenic or genotoxic effects of the compound. The results of a chromosomal aberration assay were regarded as invalid because of the strange pattern of cytotoxicity, with may be a consequence of unnoticed precipitation of the compound. An in vivo DNA binding assay showed no DNA-binding activity of 1,5-naphthylenediamine in rat liver. Overall, the mutagenic effects seen in bacteria could not be reproduced in mammalian cells. No classification for mutagenicity is proposed

Short description of key information:
In vitro:
Several in vitro genetic toxicity tests with 1,5-naphthylenediamine (Alphamin) were performed.

The test substance was tested in a Salmonella microsome test with 20-12500 µg/plate.
Doses up to 1600 µg/plate did not cause bacteriotoxic effects.
Higher doses showed bacteriotoxicity and substance precipitation occurred at the highest dose level, therefore 12500 µg/plate were not interpretable. On strains TA 1537, TA 98 and TA 100 a biologically relevant increase of revertants were found with and without metabolic activation; the effect was more pronounced with S9-mix.
The lowest effective dose was 200 µg/plate.
The genotoxicity of the test substance was found to be positive (Herbold 1988).

This result is supported by another Ames test (with and without metabolic activation):
In a preliminary experiment doses of 20-12500 µg substance/plate were tested in a Salmonella microsome test.
Doses up to 1200 µg/plate did not cause bacteriotoxic effects, higher doses revealed bacteriotoxicity and sustance precipitation occurred at doses of > 9600 µg/plate; at 12500 µg/plate the test was no longer interpretable. On strains TA 1537, TA 98 and TA 100 a biologically relevant increase in revertants was found with and without metabolic activation.
The lowest reproducible effective dose was 300 µg/plate (Herbold 1989).


Additionally the test substance was tested in 4 independent Institutes with the Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537, TA 1538 and Escherichia Coli WP2 uvrA. The doses used were: 0,3 and 3333,33 µg/plate and the experiments were performed with and without metabolical activation ( liver S-9 preparations from Aroclor 1254 -induced livers from rats, mice and syrian hamsters). The test substance was positive for TA 100(with and without metabolical activation), TA 1537 ( with and without metabolical activation) and TA 1538 (with metabolical activation). No pointmutagen effect was found in TA 1535 (with and without metabolical activatio...

Endpoint Conclusion: