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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997-07-24 to 1997-08-07
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP and guideline compliant sudy report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPP 81-2 (Acute Dermal Toxicity)
Deviations:
no
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2'-hydroxyacetophenone
EC Number:
204-288-0
EC Name:
2'-hydroxyacetophenone
Cas Number:
118-93-4
Molecular formula:
C8H8O2
IUPAC Name:
2'-hydroxyacetophenone
Details on test material:
- Name of test material (as cited in study report): 2-Hydroxyacetophenone
- Substance type: organic
- Physical state: clear pale yellow liquid
- Expiration date of the lot/batch: 30 June 1997
- Storage condition of test material: at room temperature in the dark

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Harlan lnterfauna UK Ltd, Huntingdon, Cambridgeshire, UK
- Age at study initiation: 10 - 12 wks
- Weight at study initiation: 2.2 - 2.7 kg
- Housing: individually
- Diet: ad libitum, standard laboratory rabbit diet (STANRAB (P) (SQC) Rabbit Diet from Special Diets Services)
- Water: ad libitum, tap water
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18 - 22
- Humidity (%): 47 - 79
- Air changes (per hr): 10 - 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: dorso-lumbar region (approx. 100 mm x 100 mm)
- Type of wrap if used: waterproof dressing

REMOVAL OF TEST SUBSTANCE
- Washing: with warm water (30 - 40 °C)
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amounts applied: 1.713 mL/kg bw
Duration of exposure:
24 hours
Doses:
2000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: at least twice daily; weighing was performed once a week
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality was observed.
Clinical signs:
One female showed soft to liquid feces on Days 10 and 11. No clinical signs were observed in the other animals.
Body weight:
A slight body weight loss was recorded for one male on Day 15. All other animals achieved the expected body weight gains throughout the study.
Gross pathology:
No gross internal lesions were observed during necropsy.

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information