2'-hydroxyacetophenone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-02-03 to 1988-02-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented, scientifically acceptable study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Test was performed according to the method published by Ames et al (Mutation Res., 31: 347-364, 1975)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2'-hydroxyacetophenone

EC Number:

204-288-0

EC Name:

2'-hydroxyacetophenone

Cas Number:

118-93-4

Molecular formula:

C8H8O2

IUPAC Name:

1-(2-hydroxyphenyl)ethan-1-one

Details on test material:

Name of the test substance used in the study report: o-Hydroxyacetophenon

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98, TA 100

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9 mix (Aroclor 1254 induced rat liver)

Test concentrations with justification for top dose:

Pre-test: 0.018, 0.037, 0.073, 0.146, 0.293, 0.586, 1.17, 2.34, 4.69, 9.38, 18.8, 37.5, 75, 150 µL/plate
Main test: 0.05, 0.1, 0.25, 0.5, 1, 2.5, 5, 10 µL/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Substance was soluble in DMSO.

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

without S9 mix (TA 1535, TA 100)

Controls 2

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Remarks:

without S9 mix (TA 1538, TA 98)

Controls 3

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Quinacrine mustard

Remarks:

without S9 mix (TA 1537)

Controls 4

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with S9 mix (all strains)

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Exposure duration: 48 to 72 hours

NUMBER OF REPLICATIONS: none

DETERMINATION OF CYTOTOXICITY
- Method: relative total growth

Evaluation criteria:

Strains TA 1535, TA 1537, TA 1538
Data sets will be evaluated as positive if a dose response is observed over a minimum of three test concentrations and the increase in revertants is equal to or greater than three times the solvent control value at the peak of the dose response. The solvent control value should be within the normal range for evaluating the results.

Strain TA 98, TA 100
Data sets will be evaluated as positive if a dose response is observed over a minimum of three test concentrations and the increase in revertants achieves a doubling of the solvent control value at the peak of the dose response. The solvent control value should be within the normal range for evaluating the results.

Statistics:

Not applicable.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98, TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
No confounding factors were observed during the test.

RANGE-FINDING/SCREENING STUDIES: Doses for the mutagenesis assays ware selected from a preliminary study conducted on the test material at fourteen doses from 0.018 µL per plate to 150 µL per plate using the strain TA 100. In the preliminary study, the test material was toxic to the indicator strain at 9.38 µL per plate and above as evidenced by the reduced number of revertants, and/or the appearance of micro colonies on the minimal media plates, and/or the clearing of the background lawn.

MAIN TEST: Individual plate counts for the test material are given in the table under "Any other information on results incl. tables". Since the solvent control values were out of range with strain TA 98 in the non-activation and activation assays, the tests with this strain were repeated. The second trial was discarded as it was contaminated. The results with this strain from the third trial are given in the table under "Any other information on results incl. tables". The results of the assays conducted on the test material at dose level ranging from 0.050 µL per plate to 10.0 µL per plate in the absence and presence of metabolic activation did not exhibit increased numbers of his+ revertant colonies. The positive control treatments in both the non-activation and S9 activation assays induced large increases in the revertant numbers with all the indicator strains, which demonstrated the effectiveness of the S9 activation system and the ability of the test system to detect known mutagens.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Raw data

Dose (µL/plate)	Mean number of revertant colonies/ plate with different strains of Salmonella typhimurium
	TA1535	TA1537	TA1538	TA98	TA100
	Results with S9
Spontaneous Reversion	11	7	25	36	170
Positive control	248	492	1993	1808	2365
0.05	16	7	19	38	176
0.10	19	12	28	31	148
0.25	9	10	13	40	181
0.50	13	5	24	31	164
1.0	10	10	23	29	142
2.5	12	11	30	40	126
5.0	7	2	19	15	86
10.0	1	4	10	29	60
	Results without S9
Spontaneous Reversion	26	14	17	37	169
Positive control	1409	1003	1665	1471	1207
0.05	26	11	12	30	190
0.10	20	9	12	27	149
0.25	17	5	13	32	161
0.50	23	10	15	32	183
1.0	25	11	16	30	151
2.5	34	9	19	34	144
5.0	21	4	29	24	105
10.0	21	1	8	9	36

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative