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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
Deviations:
no
GLP compliance:
yes
Type of study:
mouse local lymph node assay (LLNA)

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl oct-2-ynoate
EC Number:
203-836-6
EC Name:
Methyl oct-2-ynoate
Cas Number:
111-12-6
Molecular formula:
C9H14O2
IUPAC Name:
methyl oct-2-ynoate
Test material form:
liquid

In vivo test system

Test animals

Species:
mouse
Strain:
CBA/Ca
Sex:
female

Study design: in vivo (LLNA)

Vehicle:
other: ethanol/diethylphthalate
Concentration:
A volume of approximately 25 ul of 0.05, 0.1, 0.25, 0.5 or 1.0% w/v test material in 1:3 ethanol/diethylphthalate (vehicle) was applied topically to the dorsum of each ear lobe (left and right) on three consecutive days.
No. of animals per dose:
4
Details on study design:
Animals were divided into 6 groups consisting of 4 animals in each group. Animals were 8-12 weeks of age. Groups included 1 control (vehicle) group and 5 test groups.

Results and discussion

In vivo (LLNA)

Results
Key result
Parameter:
EC3
Value:
0.45

Applicant's summary and conclusion

Interpretation of results:
Category 1A (indication of significant skin sensitising potential) based on GHS criteria
Executive summary:

Under the conditions of the study, Folione in 1:3 EtOH:DEP is a potential skin sensitizer with an EC3 value of 0.45% (112.5 ug/cm2).