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EC number: 942-380-9 | CAS number: 131796-64-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (3-acetyloxy-2,2-dimethylpropyl) acetate
- EC Number:
- 826-122-1
- Cas Number:
- 13431-57-7
- Molecular formula:
- C9 H16 O4
- IUPAC Name:
- (3-acetyloxy-2,2-dimethylpropyl) acetate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: BSCL20180222-2105
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature
- Water Solubility: 814 mg/L (20°C, pH 5.8)
- The test item was a clear colorless apparently homogeneous liquid.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A certain amount of test substance was added in a certain volume of test water and stirred (via magnetic stirrer) in the dark for l h (the vortex depth was 1/3 of that of the mixture column) until it dissolved, to prepare a stock solution with a nominal concentration of 120 mg/L. The test solutions were prepared by dilution of the stock solution with test water. The frequency of the renewal of the stock solution and the test solutions were both 48h during the definitive test.
Test organisms
- Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
The test was performed with Gobiocypris rarus which were bred in Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement (ID:GR-SAPM-180423).
ACCLIMATION
Before being used in the test, all fish were acclimatized in the following test conditions for more than 14 days:
Water: the test water used was tap water dechlorinated by activated carbon and sterilized by UV lamp;
Temperature: 21-25tC and constant within a range of 2C.
pH: 6.0-8.5;
Dissolved oxygen concentration: > 80% of air saturation value (ASV);
Photoperiad: Lightioark = 12 hours/12 hours;
Feeding: fish were fed with brine shrimp flake (O.S.l, USA) daily until 24 hours before the test was started.
Length was 2.4 ± 0.1 cm (Mean ± SD) and weight was 0.27±0.01 g (Mean ± SD) at the end of the test. The age of the test fish was approximately seven months old, before the test they were not medically treated.
FEEDING DURING TEST : none
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21.7 °C - 23.7 °C
- pH:
- 6.0 - 8.5
- Dissolved oxygen:
- 61.3% - 98.9%
- Nominal and measured concentrations:
- Nominal: 0, 42 mg/L, 55 mg/L, 71 mg/L, 92 mg/L and 120 mg/L
Measured: 0, 38.1 mg/L, 50.4 mg/L, 67.1 mg/L, 91.0 mg/L and 115 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10L glass vessels with seals containing 9 L test solution
Loading rate: about 0.2g fish/litre
Aeration: no
- Renewal rate of test solution (frequency/flow rate): 48h
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, dechlorinated by activated carbon and sterilized by UV lamp, was used. The water was aerated continuously over 24h.
OTHER TEST CONDITIONS
- Light intensity: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
At 3, 24, 48, 72 and 96 h, the mortalities of the fish were recorded, and observations on individual behaviour were performed. Meanwhile, measurements of pH, dissolved oxygen and temperature were carried out and recorded daily.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 d
- Dose descriptor:
- LC50
- Effect conc.:
- 74.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The maximum mean measured concentration causing no mortality and the minimum mean measured concentration causing 100 percent mortality within the period of the test was 50.4 mg/L and 91.0 mgIL, respectively. The results above were expressed relative to the geometric means of the measured concentrations.
- Reported statistics and error estimates:
- The mortalities were plotted against each test concentration (geometric mean measured) and control for each exposure period (24h, 48h, 72h and 96h). The 24, 48 and 72 hours LC50 were calculated using the method of Linear Interpolation. The 96 hours LC50 with 95% confidence limits were calculated using the method of Spearman-Karber. The data analysis was carried out with software TOXCALO-Toxicity Data Analysis Software (v5.0.32).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h LC50 value for Pyranyl acetate to Gobiocypris rarus was 74.9 mgIL and 95 percent confidence limits ranged from 69.3 mg/L to 81.0 mg/L.
No mortality was observed in the control during the test. Acceptable exposure conditions were maintained throughout the test. The dissolved oxygen concentrations in test solutions were between 61.3%- 98.9%ASV, which was greater than 60.0% of the air saturation value, throughout the test. During the test, the measured concentrations of the test substance in each group varied less than 20% during each renewal period (-7.25%-(-0.766%)), even those of the freshly prepared solutions varied within ± 20% of their arithmetic mean measured one (-0.963%-0.963%).
The test fulfilled the above requirements and was therefore considered to be valid.
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