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EC number: 209-812-1 | CAS number: 593-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Remarks:
- Data are included only to justify the read-across approach of the dossier
- Adequacy of study:
- supporting study
- Study period:
- end of 1988 - early 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Guanidine nitrate
- IUPAC Name:
- Guanidine nitrate
- Reference substance name:
- Guanidinium nitrate
- EC Number:
- 208-060-1
- EC Name:
- Guanidinium nitrate
- Cas Number:
- 506-93-4
- Molecular formula:
- CH6N4O3
- IUPAC Name:
- amino(imino)methanaminium nitrate
- Details on test material:
- - Name of test material (as cited in study report): Guanidine Nitrate
- Physical state: solid
- Analytical purity: 99,3 %
- Lot/batch No.: 010488
- Stability under test conditions: stated to be very stable when stored in dry conditions
- Storage condition of test material: in a container at ambient temperature and humidity in the dark
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD(SD)BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River (UK) Ltd., Margate
- Age at study initiation: 6 to 8 weeks
- Weight at study initiation: 180 to 200 g
- Fasting period before study: not mentioned
- Housing: single room, groups of 5 by sex, grid-floor stainless steel cages
- Diet (e.g. ad libitum): not mentioned
- Water (e.g. ad libitum):not mentioned
- Acclimation period: at least 11 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 to 25 °C, at several occasions 18 to 29 °C
- Humidity (%): 40 to 70 %
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- head only
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: cylindrical aluminium exposure chamber
- Exposure chamber volume: 40 L
- Method of holding animals in test chamber: restrained in plastic tubes, nares only project into the chamber
- Source and rate of air: clean dry filtered air, diluent air flow 10.0 to 10.3 L/min
- Method of conditioning air: compression
- System of generating particulates/aerosols: Wright dust feed generator
- Method of particle size determination: Delcron CS5 Cascade Impactor with 5 separation stages (0.5, 1.0, 2.0, 4.0, 8.0 μm)
- Temperature, humidity in air chamber: 18 to 20 °C, 37 to 55 % humidity
TEST ATMOSPHERE
- Brief description of analytical method used: For the determination of the nominal concentration, the total weight of test article used and the volume Q of diluent air used to generate the test atmosphere were recorded and the nominal concentration of the test article in the exposure chamber was calculated as follows:
Nominal concentration (mg/L) = weight of test article used (mg) / flowrate (L/min) x duration (min)
- Samples taken from breathing zone: not specified
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: particle size analysis was performed
- MMAD (Mass median aerodynamic diameter): 5.52 μm - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- The study was performed as a limit test.
mean measured concentration: 0.853 mg/L
nominal concentration: 17.273 mg/L - No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: hourly during exposure and for the remainder of the exposure day then daily for 14 days
- Frtequency of weighing: day of dosing, days 8 and 15 of the study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, lung weight - Statistics:
- means and standard deviations calculated where appropriate
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 0.853 mg/L air (analytical)
- Exp. duration:
- 4 h
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 17.273 mg/L air (nominal)
- Exp. duration:
- 4 h
- Mortality:
- There were no deaths.
- Clinical signs:
- other: Slight clinical signs of staining and wetting of the fur in the test group disappeared by day 4.
- Body weight:
- There was no treatment-related effect on body weight.
- Gross pathology:
- There were no treatment-related changes in lung weight or in macroscopic appearance at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Conclusions:
- The LC 50 of Guanidine Nitrate was determined to be > 0.853 mg/L air (analytical) in this study. This concentration was the maximum possible under conditions of this study, generated from a nominal concentration of 17.273 mg/L. No animal died and no treatment related clinical signs were observed.
- Executive summary:
In an acute inhalation toxicity study performed similar to OECD Guideline 403 5 male and 5 female young adult Crl:CD(SD)BR rats were exposed by inhalation route to micronized Guanidine Nitrate (99.3 % a.i.) for 4 hours to head only at a chamber concentration of 0.853 mg/L. The corresponding nominal concentration was 17.273 mg/L and the mass median aerodynamic diameter of the particles in the atmosphere was 5.52 µm. The nominal concentration was the maximum practical for the generator used and the resulting analytical concentration the maximum possible under conditions of this study. Animals then were observed for 14 days.
LC50 Combined = > 0.853 mg/L air (analytical)
Combined = > 17.273 mg/L air (nominal)
No animals died and there were no treatment related clinical signs, necropsy findings( including lung weights) or changes in body weight, exceptof staining and wetting of the fur, which disappeared by day 4.
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