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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from computational model developed by USEPA
Justification for type of information:
Data is from computational model developed by USEPA
Qualifier:
according to
Guideline:
other: Modeling database
Principles of method if other than guideline:
ECOSAR estimates log Kow values using the US EPA's KOWWIN Program for aquatic toxicity.Toxicity of a chemical was measured on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program based upon the octanol /water partition coefficient (Kow). Data from ECOSAR class,neutral organics was used.

The estimation methods in EPI Suite™ have been developed by government, academic, and private sector researchers over many years and represent some of the best techniques currently available. The intended application domain of EPI Suite is organic chemicals, and inorganic as well as organometallic chemicals generally can be considered outside the domain.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Fish
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.945 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours LC50 was estimated to be 0.945 mg/l on fish for test substance with mortality effects. Thus based on this value it can be concluded that the substance is highly toxic and can be classified as Aquatic chronic 1 as per the criteria of CLP regulation.
Executive summary:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours LC50 was estimated to be 0.945 mg/l on fish for substance test substance with mortality effects. Thus based on this value it can be concluded that the substance is highly toxic and can  be classified as Aquatic acute 1 as per the criteria of CLP regulation.

Description of key information

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (2017), the 96 hours LC50 was estimated to be 0.945 mg/l on fish for substance test chemical with mortality effects. Thus based on this value it can be concluded that the test chemical is toxic to fish and can  be classified as 'Aquatic acute 1' as per the criteria of CLP regulation.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.945 mg/L

Additional information

Predicted data of the target chemical and various experimental studies for its read across substance was reviewed for the short term toxicity to fish end point which is summarized as below:

 

In a prediction done using the EPI Suite ECOSAR version 1.10 (2017), the 96 hours LC50 was estimated to be 0.945 mg/l on fish for substance test substance with mortality effects.

 

Another Prediction done using Danish QSAR database (2018) in which the average value of both models i.e Leadscope and SciMatics SciQSAR model were presented in Battery model. On the basis of this, 96 hours LC50 was estimated to be 0.44 mg/l on Pimephales promelas for test substance with mortality effects.

 

In a supporting weight of evidence study from peer reviewed journal (Kuo-HsiungL et al, 2002), short term toxicity test was performed to evaluate the effect of test material to fishCyprinus carpio. The test animals were fed with yeast in a 224 (diameter), 46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carps of 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical i.e, 10, 50, 100, 200, and 500 mg/l. After 48 h of exposure period, median lethal concentration (LC50) values were calculated by using trimmed Spearman Karber method. The median lethal concentration (LC50) of test material onCyprinus carpioafter 48 h was observed to be 0.022 mg/l.

For the test chemical, another short term toxicity test was performed to evaluate the effect of test material to fish Cyprinus carpio (Kuo-HsiungL et al, 2002). The test procedure and conditions were similar as mentioned above. The median lethal concentration (LC50) of test material on Cyprinus carpio after 48 h was observed to be 0.045 mg/l.

On the basis of the available information, test chemical is toxic to aquatic fish and can be classified as ‘Aquatic acute 1 category’ as per the criteria of CLP regulation.