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EC number: 914-471-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From 03 DEC 2001 to 16 FEB 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to relevant guideline and compliant to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Vers. APR 1984, Part I, 24h acute immobilization test
- Deviations:
- yes
- Remarks:
- exposure period 48h
- GLP compliance:
- yes
Test material
- Reference substance name:
- Montan waxes, type CaV 102
- IUPAC Name:
- Montan waxes, type CaV 102
- Details on test material:
- "Licomont CaV 102"
active content: 100 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- According to "Guidance Document On Aquatic Toxicity Testing Of Difficult Substances And Mixtures" of the OECD, Environmental Health and Safety Publications, No. 23 - 3.1 Poorly water-soluble substances.
A definite amount of the test substance (10 mg; 100 mg; 10 g etc.) was filled into measuring flask and the total volume made up with water at 21 +/- 1 deg C.
The solution was shaken during 24 hours. The test substance was not soluble in water.
Shaking produced a dispersion of the test substance. Then the solution was filtrated. The hydrophobic properties of "Licomont CaV 102" didn't allow an investigation of higher concentrations (more than 10 g/L).
The test bottles were conditioned about 15 minutes before the test was started.
The pH value and oxygen concentration were measured at the beginning and the end of the test.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS
Supplier: Umweltbundesamt, 12307 Berlin
Feeding: daily with an freshwater-alga-suspension (Scenedesmus subspicatus); illumination diffuse, full time Temperature: ca. 20 deg C
Age of Daphnia for the tests: 6 - 24 hours old
Not feeding during test, performance in the dark (20 animals per concentration)
Dilution water: produced synthetically, pH value 7.8; ISO-Test water: 25 mL/L each of solutions (CaCl2 * 2 H20, MgSO4 * 7H20, NaHCO3 and KCI)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- ISO Test water as recommended by the guideline
- Test temperature:
- 20 +/- 1 deg C
- pH:
- Control
test start: 7.8; test stop: 7.4
treatment groups:
test start: 7.5 - 7.7
test stop: 7.3-7.5 - Dissolved oxygen:
- [mg/L]
Control:
test start: 6.8; test stop: 5.6;
treatment groups:
test start: 6.0-6.5;
test stop: 5.6 - 5.7; - Nominal and measured concentrations:
- Applied nominal concentrations: 5.2, 10.1, 100, 1000 and 10000 mg/L
The test item was insoluble. No analytical determination; test solutions were prepared with nominal concentrations and filtered before application. - Details on test conditions:
- The investigation of acute immobilization was performed as described in the OECD 202 method. The species Daphnia magna STRAUS were put into the test solution of chosen concentrations.
20 Daphnia were used for each test concentration in a test volume of 40 mL (four groups of five animals each). The animals were inspected after 24 and 48 hours. The inhibitory effect on the swimming capability of Daphnia was the basic data for evaluating toxic effects. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 10.1 - <= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In the control no immobilized daphnids were observed. With the highest treatment concentration of 10 g/L, 30% of the daphnids were immobilized.
- Results with reference substance (positive control):
- Potassium dichromate:
24h-EC50 = 1.0 mg/L
mortality of the Daphnia in the control solution at the end of the test: 5 % (reference value < 10 %)
dissolved oxygen concentration 62 % of the air saturation (reference value > 60 %) - Reported statistics and error estimates:
- A Probit analysis is not possible with these results. Only a small amount of the Daphnia were immobilized at different concentrations, but the effect is not greater than 30 per cent even in the highest concentration 10 g/L.
Any other information on results incl. tables
Details on results:
Concentration of |
No swimming |
No swimming |
pH value |
pH value |
O2(mg/L) |
O2(mg/L) after test |
control solution |
0 |
0 |
7.8 |
7.4 |
6.8 |
5.6 |
5.2 |
0 |
0 |
7.7 |
7.5 |
6.5 |
5.7 |
10.1 |
0 |
10 |
7.6 |
7.3 |
6.0 |
5.7 |
100 |
0 |
10 |
7.5 |
7.4 |
6.0 |
5.7 |
1000 |
0 |
30 |
7.6 |
7.4 |
6.0 |
5.6 |
10000 |
0 |
30 |
7.5 |
7.4 |
6.3 |
5.6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute toxicity test with the submission substance on Daphnia magna according to OECD 202 the EC50 (24 and 48 h) for immobilisation was > 10 g/L.
- Executive summary:
In an acute toxicity test with the submission substance on Daphnia magna according to OECD 202 and compliant to GLP (reliability category 1) no immobilization was observed in the control. In the treatment groups with the highest tested concentration of nominal 10 g/L a maximum of 30% of daphnids were immobilized after 48h.
EC10 (48h) >= 10.1 <= 100 mg/L
EC50 (24 and 48 h) > 10 g/L
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