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Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 1993-06-08 to 1993-07-06
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: BBA-Richtlinie BBA-CP 411 3/90
Qualifier:
equivalent or similar to guideline
Guideline:
ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
Principles of method if other than guideline:
Examination of the reproductive performance of Collembolen for the NOEC value determination after 28 days.
GLP compliance:
yes (incl. QA statement)
Application method:
soil

Test material

Constituent 1
Reference substance name:
dibenzyltoluene
IUPAC Name:
dibenzyltoluene
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): Marlotherm S
- Substance type: pure active substance
- Physical state: yellow liquid
- Lot/batch No.: 200 l barrel from August 21, 1991
- Stability under test conditions: unlimited
- Storage condition of test material: no data
Specific details on test material used for the study:
The purity of the material used for the test was lower than the purity of the actual material. It contained ca. 26% of isomers of dimethyl tribenzenes. However, based on comparable basic structure of the impurities and the assumption that any toxic effects would increase with higher molecular weight and lipophilicity, we regard that the studies conducted with the lower purity material being "worst case" and can be used for the evaluation of the actual product.

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
TEST BATCH AND ITS PREPARATION
- Vehicle, solvent: no vehicle/solvent used
- Procedure: 1 application at the beginning of the test, the test substance was weighed in silica sand and then mixed with the other soil components. The soil substrate was distributed to the test vessels and the test organisms were set in immediately after.

Test organisms

Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)
Details on test organisms:
TEST ORGANISMS
- Strain: Folsomia candida (Willem)
- Supplier: Battelle Europe e.V., Frankfurt a.M., Germany
- Wild caught: no
- Breeding method: at 20-22°C and diffuse light (approx. 2.2 µmol/m²s, natural diurnal rhythm) in transparent round polyethylene vessels (7 cm diameter, 7.5 cm height), culture substrate consisting of 8 parts gypsum and 1 part charcoal (40 % moisture), feeding 1 ¿ 2 times per week, approx. 10 mg grained dry yeast (Dr. Oetker, Bielefeld, Germany) per breeding container
- Age: 10 ¿ 12 days old
- Substrate: 69 % silica sand, 20 % kaoline, 10 % turf, 1 % lime, moistened with aerated, dechlorinated tap water up to a moisture of approx. 25 %
- Feeding: 2 mg grained dry yeast each attempt at the beginning of the test and after 14 days
- Pretreatment: none

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
28 d
Post exposure observation period:
two times per week.

Test conditions

Test temperature:
18 - 25 °C
pH (if soil or dung study):
7.29 (1000 mg/kg dw) ¿ 7.46 (control) at the beginning of the test; 7.91 (control) ¿ 7.95 (100 mg/kg dw), resp. 7.93 (1000 mg/kg dw) at the end of the test
- Adjustment of pH: not adjusted
Humidity:
approx. 25 %
Photoperiod and lighting:
not mentioned
Details on test conditions:
- Exposure vessel type: 250 ml glass flasks with screw top, 2 times per week the test vessels were opened 2 minutes for ventilation
- Number of replicates, collembola per replicate: 4 replicates, 10 collembola per replicate, 1 replicate per concentration without collembola for determination of soil parameter
Nominal and measured concentrations:
Nominal: 0 (control), 10, 32, 100, 320 and 1000 mg/kg dry weight
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
320 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
320 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
1 000 mg/kg soil ww
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
RESULTS: EXPOSED
- Nominal concentrations: 10, 32, 100, 320, 1000 mg/kg dw
- Effect data:
Mortality of adults after 28 days (mean values):
10 mg/kg / 22.5 %
32 mg/kg / 12.5 %
100 mg/kg / 22.5 %
320 mg/kg / 55* %
1000 mg/kg / 63.5* %
Number of juveniles after 28 days (mean values):
10 mg/kg / 726
32 mg/kg / 785
100 mg/kg / 752
320 mg/kg / 631
1000 mg/kg / 424*
* significant deviation (DUNNET¿s-test)
RESULTS: CONTROL
- Effect data:
Mortality: 12.5 %
Number of juveniles: 726
RESULTS: TEST WITH REFERENCE SUBSTANCE
No reference substance tested
Results with reference substance (positive control):
not performed
Reported statistics and error estimates:
statistics procedure according DUNNET with program SigmaStat 5.0.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The mortality of in the test groups with 320 mg/kg and 1000 mg/kg dry soil was significantly above the control groups. In the highest test concentration the mortality was 62.5 %. In the highest concentration only dead juveniles was found. No effects on reproduction were found in the 320 mg/kg dry soil test group.

mortality: NOEC = 100 mg/kg dry soil, LOEC = 320 mg/kg dry soil

reprodution: NOEC = 320 mg/kg dry soil, LOEC = 1000 mg/kg dry soil

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