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EC number: 205-622-8 | CAS number: 144-29-6
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- Endpoint summary
- Appearance / physical state / colour
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- Density
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Toxicological Summary
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: refer below principle
- Principles of method if other than guideline:
- In vitro toxicity study of Piperazine citrate on Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Piperazine citrate / tripiperazine dicitrate- Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid
- Analytical monitoring:
- not specified
- Details on sampling:
- Details on sampling- Concentrations: 200 mg/l
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: Gyrodactylus sp. were obtained from rainbow trout placed in Petri dishes containing different concentrations of anthelmintic - Controls: Yes - Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Test organisms (species):
- other: G. Malmberg as Gyrodactylus salaris
- Details on inoculum:
- - Laboratory culture: One sample obtained from infested rainbow trout
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 60 min
- Remarks on exposure duration:
- 30 and 60 min. study
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- nominal and measured concentration : Measured concentration
- Details on test conditions:
- TEST SYSTEM- Test vessel: Petri Dishes- Type (delete if not applicable): open - No. of organisms per vessel: 6 to 8 specimens - No. of vessels per concentration (replicates): 2 - No. of vessels per control (replicates): 1 - No. of vessels per vehicle control (replicates): 1 EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Number of dead (immobile) helminths.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 60 min
- Dose descriptor:
- EC0
- Effect conc.:
- 200 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Number of dead (immobile) Gyrodactylus salaries
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate.
- Executive summary:
In an in - vitro toxicity study, Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate in the concentration of 0 and 200 mg/l. no effect were observed on Percentage of reduction and number of dead (immobile) Gyrodactylus salaries. Therefore, EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate.
Reference
Oncorhynchus mykiss. Treatment against Gyrodactylus sp. Results obtained under in vitro conditions for the drugs examined. Doses expressed as mg I-'; length of exposure, in min; percentage of reduction gives the number of live Gyrodactylus
Drug | Dose mg/l | Time of exposure (min) | Percentage of reduction | Nos. of Gyrodactylus sp. | |
total | Dead | ||||
Piperazine citrate | 200 | 60 | 0 | 8 | 0 |
200 | 30 | 0 | 8 | 0 |
relative to the initial number; also listed are the number of Gyrodactylusinitially tested and the number of dead worms counted at the end of the treatment.
Control lots remained unaffected after 60 min in drug-free water under conditions identical to those in the treated lots
Description of key information
EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate. Based on this result it may be concluded that the substance Piperazine citrate (CAS no. 144-29-6) is non toxic to micro organisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 200 mg/L
Additional information
Toxicity to micro organisms was assessed for the target compound Piperazine citrate (CAS no. 144-29-6) and its structurally related read- across substance by reviewing a number of studies. The summary of the results are presented below as weight of evidence approach:
Key study from reviewed journal DISEASES OF AQUATIC ORGANISMS, Vol. 10: 39-43, 1991 indicate that thein - vitro toxicity study, Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate in the concentration of 0 and 200 mg/l. no effect were observed on Percentage of reduction and number of dead (immobile) Gyrodactylus salaries. Therefore, EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate.
Various supporting studies of the read across compound piperazine (CAS no. 110-85-0) obtained from European Union Risk Assessment Report – PIPERAZINE, 3rd Priority List Volume: 56; final Report, 2005 were reviewed and are summarised as below:
The respiration inhibition of nitrifying bacteria was studied in a two hours study (Balk and Meuwsen, 1989c). No guidelines were referred to. The nominal test concentrations were 410,750 and 1,350 mg/L. The test temperature was 20°C, and the pH was kept neutral with HCl.
Further, the inhibition of cell multiplication of Pseudomonas putida was investigated during 18 hours in a study generally in accordance with an ISO Guideline (van Ginkel, 1989). The nominal test concentrations of piperazine were 62.5, 125, 250, 500 and 1,000 mg/L. Test temperature was 25°C, pH was adjusted to neutral by means of titration with H2SO4. Cell density was determined photometrically in single cultures at the beginning of the incubation and after 18 hours. No effect on cell multiplication was observed in any of the tested concentrations compared to the controls. NOEC was determined to be >1,000 mg/L (nominal concentration).
Similarly, an activated sludge respiration inhibition test was performed according to EEC Guidelines (OECD 209) (van Ginkel and Stroo, 1989). Homogenised sludge (0.46 g dw/L) was incubated at 20°C and pH 7.4-7.8 for 30 minutes with nominal test concentrations of 20, 60,180, 540 and 1,620 mg/L plus control. The oxygen depletion was measured in single samples using an oxygen electrode. At the highest test concentration, respiration inhibition was 16% compared to the control. NOEC was determined to be 540 mg/L.
Based on above results for target chemical as well as its read across substance and by applying weight of evidence approach it may be concluded that the substance Piperazine citrate (CAS no. 144-29-6) is non toxic to micro organisms.
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