3-(2-ethylhexyloxy)propylamine

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-(2-ethylhexyloxy)propylamine

EC Number:

226-420-6

EC Name:

3-(2-ethylhexyloxy)propylamine

Cas Number:

5397-31-9

Molecular formula:

C11H25NO

IUPAC Name:

3-[(2-ethylhexyl)oxy]propan-1-amine

Details on test material:

- Batch no.: 52-4155
- Physical state: liquid
- Water solubility: >100 < 1000 mg/L
- Colour: slightly yellowish, clear
- Instability against: acid, alkali

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION
- 2000 mg/L completely soluble after stiring for 30 min and 2 hours (dispersion)

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

- Origin: activated sludge from laboratory wastewater plant treating municipal sewage
- Concentration of dry substance: 1 g/L
- Preparation of inoculum: inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +- 2°C

pH:

7.1 - 7.5

Nominal and measured concentrations:

- Concentrations tested (nominal): 1000, 496, 248 and 100 mg/L plus control

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Test volume: 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- Inoculum: 50 ml/vessel
- pH was adjusted

FURTHER TEST CONDITIONS
- Oxygen concentration during aeration: > 2.5 mg/L
- Oxygen concentration immediately before measurement: > 6.5 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

ca. 73 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

ca. 280 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50 (30 min) = ca. 10 mg/L