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EC number: 202-044-8 | CAS number: 91-15-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study is comparable to OECD 401 with acceptable restrictions mostly due to reduced reporting in times before GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 969
- Report date:
- 1969
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Phthalonitrile
- EC Number:
- 202-044-8
- EC Name:
- Phthalonitrile
- Cas Number:
- 91-15-6
- Molecular formula:
- C8H4N2
- IUPAC Name:
- benzene-1,2-dicarbonitrile
- Details on test material:
- I- Name of test material (as cited in study report): phthalonitrile
- Analytical purity: pure
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: US
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: breeder
- Mean weight at study initiation: 200 +- 23 g (male), 156 +- 8 (female)
ENVIRONMENTAL CONDITIONS
not reported
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: aqueous emulsion with traganth
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0.5% - 2%
MAXIMUM DOSE VOLUME APPLIED: 16 mL/kg = maximum 3.7 mL per male and 2.6 mL per female animal, respectively - Doses:
- 50, 100, 125, 160 and 200 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 7/14 days
- Frequency of observations and weighing: Weighing was performed only at the beginning of the study. Observation of clinical signs was several times on the day of administration and once daily afterwards except on weekends and on holidays.
- Necropsy of survivors performed: yes - Statistics:
- Not performed.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 125 mg/kg bw
- Mortality:
- 200 mg/kg: 10/10 animals died within 24 hrs after application
160 mg/kg: 8/10 animals died within 24 hrs after application
125 mg/kg: 4/10 animals died within 24 hrs after application
100 mg/kg: 1/10 animals died within 24 hrs after application
50 mg/kg: no mortality - Clinical signs:
- other: 100 - 200 mg/kg: directly after application: agitation, accelerated and partly intermittent respiration, clonic convulsions, cry of pain, Straub tail phenomenon ("Morphinschwanz"), oral secretion, ruffled, fluffed fur. Symptoms started to decrease slowly
- Gross pathology:
- Animals that died
Strong dilatation of the gastrointestinal tract, clear test substance odor, kidney congestion, 1x bloody congested lung, serous or bloody serous smeared snouts in all animals, 1x thorax tissue layers filled with liquid , 4x terminal blood congestion of the lung and the liver, 1x lung edema
Sacrificed animals
Nothing abnormal found
Any other information on results incl. tables
LC0 = 50 mg/kg
Applicant's summary and conclusion
- Executive summary:
The study was comparably performed according to OECD 401 with acceptable restrictions mostly due to reduced reporting in times before GLP. Groups of 5 rats per sex and dose were administered 50, 100, 125, 160 and 200 mg/kg of the test substance. The acute LD50 for male and female rats is ca. 125 mg/kg. Clonic convulsions, Straub tail phenomenon, oral secretion, waddling gait and lung edema in one animal that died were observed.
Conclusion
O-phthalonitrile is toxic after acute oral application (R25 following EU classification requirements and Cat. 3 following GHS criteria).
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