Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Ames Test; HPRT test, in vivo CA and occupational worker screening: negative

Additional information

ortho-Phthalodinitril was tested in the standard plate test and in the pre-incubation test with and without metabolic activation in S. typhimurium TA98, TA100, TA1535 and TA1537 at dose levels of 20 -5000 and 4 - 2500 µg/plate, respectively. The study was conducted similar to the OECD Guideline 471 and is reliable with restrictions. No increase in the number of revertants was detected in any strain with and without MA. Vehicle controls and positive controls were valid. Cytotoxicity was found depending on the strain and metabolic activation. Precipitation was reported.

Beyond this, no mutagenicity was found for ortho-Phthalonitril in an in-vitro mamalian cell genotoxicity study (HPRT Test). An in-vivo chromosomal aberration test did not find clastogenic activity. No hint for any mutagenic properties was found in human lycocytes in a occupational health investigation.

Justification for classification or non-classification

No classification suggested for mutagenicity as criteria of regulation 1272/2008/EC are not met.