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EC number: 285-249-5 | CAS number: 85049-76-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Non ready biodegradable.
Key value for chemical safety assessment
Additional information
No data on Acid Green 104 was available, thus a read-across approach was adopted. In particular, available data on Similar Substance 01 and Similar Substance 02, i.e. cobalt complexes with 2 phenyl-azo-naphtyl ligands functionalised by similar groups, were used.
As for Similar Substance 01, biodegradablility potential was assessed in an experimental study conducted according to OECD 301B. The initial test substance concentration was 20 mg/l based on TOC. After an incubation time of 28 days, less than 10 % of the test compound was degraded. Thus, the substance was considered as not readily biodegradable and regarded as poorly biodegradable.
As for Similar Substance 02, biochemical oxygen demand of a seed of microorganisms after 5 days incubation at 20 °C (BOD5) was 0 mg O2/g; chemical oxygen demand (COD), measured as the amount of oxygen needed to oxidise test substance, was 493 mg O2/g. On these bases, a BOD5/COD equal to 0 was obtained. This was interpreted as lack of rapid degradation potential.
Moreover, no evidence of degradation activity was noted in a inherent biodegradability test. Thus, the test was finished after 21 days instead of 28 days.
In all cases, the validity of the tests was confirmed by controls conducted with proper reference substances.
Further BOD5 and COD values were available for various samples with lower purities. As no details were available, key values are reported in the table below. Overall, these results confirm the lack of rapid degradation potential for the test substance.
| FAT | % dye | BOD5 | COD | BOD5/COD | pH | date |
| FAT 20036/A | 42% | 130 | 994 | 0.13 | 6.6 | 1974 |
| FAT 20036/B | 75% | 17 | 1085 | 0.02 | 8.9 | 1978 |
| FAT 20036/C | 63% | 0 | 811 | 0.0 | 7.3 | 1980 |
| FAT 20036/D | 71.8% | 16 | 757 | 0.02 | 8.8 | 1982 |
Available information indicated each FAT as composed by dye and coupage, typically sodium lignosulfonate, water, sodium sulfate. Such species were expected to have no impact on the toxicological profile.
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