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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly weighed into the test vessels.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF

HOUSING CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: ca. 5 µE/m²s at a wavelength of 400-750 nm

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.78 mmol/L
Test temperature:
ca. 21 °C
pH:
7.9 - 8.2
Dissolved oxygen:
Study initiation: 9.00 - 9.25 mg/L
Study end: 8.51 - 8.76 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 62.5, 125, 250, 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, fill volume: centrifuge tubes with flat bottom, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water (conductivity < 0.05 µS/cm) aerated with oil-free air for 24 h
- Alkalinity: 0.92 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 660 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content after 0 and 48 hours

OTHER TEST CONDITIONS
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED: mobility after 0, 3, 6, 24, 48 hours

Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 130 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: none
Validity criteria fulfilled:
yes

Description of key information

EC50(48h) > 130 mg/L (nominal, active ingredient) for Daphnia magna (OECD 202)

Key value for chemical safety assessment

Additional information

One study investigating the short-term toxicity of the test substance to aquatic invertebrates is available. The study was performed according to EU method C.2 using Daphnia magna as test organism (BASF 1989). Up to the highest test concentration of 500 mg product/L (= 130 mg a.i./L) no effects were observed. Thus, the 48h-EC50 is determeined to be > 130 mg a.i./L based on nominal concentrations.