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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no GLP, 30 min. incubation time
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: municipal WWTP Deidesheim (sampled 6.6.1989)
- Initial biomass concentration: 4.7 g/L
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min
Nominal and measured concentrations:
Nominal: 1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Key result
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
>= 260 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
At 1000 mg/l stimulation of the respiration (5%) was observed.
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: EC50 = 15 mg/L
Validity criteria fulfilled:
not specified

Description of key information

EC0(30 min) >= 260 mg/L (nominal, active ingredient) for activated sludge (ISO8192, part B)

Key value for chemical safety assessment

Additional information

One study investigating the toxicity of the test substance to aquatic microorganisms is available. The study was performed according to ISO guideline 8192 using activated sludge as inoculum (Wonner 1989).

1000 mg product/L (= 260 mg a.i./L) were tested in a limit test. After an incubation time of 30 minutes, no respiration inhibition was obsereved in the treatment compared to the control. Thus, the 30 min-EC0 is determined to be >= 260 mg a.i./L based on nominal concentrations.