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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
other information
Study period:
Dec 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well reported guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Hydrocortisone 21-acetate
EC Number:
200-004-4
EC Name:
Hydrocortisone 21-acetate
Cas Number:
50-03-3
Molecular formula:
C23H32O6
IUPAC Name:
11,17-dihydroxy-3,20-dioxopregn-4-en-21-yl acetate
Details on test material:
- Name of test material (as cited in study report): hydrocortisone-21-acetate (ZK 5192)
- Analytical purity: 99.4%
- Lot/batch No.: 20048246
- Expiration date of the lot/batch: Sep 1995

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

No inhibiton of respiration due to hydrocortisone-21 -acetate was observed at any concentration tested.

The reference substance showed a EC50 of 7.9 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
the EC10/EC50 of hydrocortison-21 -acetate to activated sludge microorganisms is higher than 100 mg/L
Executive summary:

The toxicity to activated sludge miccroorganisms was studied in a respiration inhibition test according to OECD 209. The microorganisms were incubated for 30 min with the test compound at a nominal loading of 100 mg/L, which was above the solubility level. No inhibiton of respiration was observed. Therefore, the EC10/EC50 of hydrocortison-21 -acetate to activated sludge microorganisms is higher than 100 mg/L.