Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-553-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Remarks:
- HPLC estimation method
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 July 2016 to 8 July 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (version 2001)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- (version 2008)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Radiolabelling:
- no
- Test temperature:
- 25 ± 0.5 °C.
- Details on study design: HPLC method:
- TEST ITEM PREPARATION
20.1 mg test substance were weighed into a 10-mL flask and filled up to 10.05 mL with methanol. 25 μL of the resulting solution with a concentration of 2 g/L, were mixed with 525 μL Methanol and 450 μL water and used for measurement (final concentration 50 mg/L). The pH value of this solution was determined to be 6 - 7 using indicator paper.
TEST SYSTEM
High performance Liquid Chromatography (HPLC)
- Degasser: Agilent G1379A
- Quaternary pump: Agilent G1311A
Autosampler: Agilent G1313A
- Column compartment: Agilent G1316A
- UV Detector: Agilent MWD G1365B
- Software: ChromeleonTM, Version 6.80 SR11d Bulild 3302
HPLC METHOD PARAMETERS
- Eluent isocratic methanol/water 55/45 % (v/v), degassed.
- Flow rate: 0.8 mL/min
- Injection volume: 10 μL
- Column temperature: 25 ± 0.5 °C.
- Wavelenght for evaluation: 210 nm
PERFORMANCE OF THE STUDY
- Pre-test: A solution of the test item in eluent was prepared and injected in order to check detectability and to find an approximate range for log Koc. In the preliminary test, a solution of the test item with a concentration of 50 mg/L was injected in order to check retention time and the signal height. The first injection was run with pump mixed eluent (from pure methanol and pure water) which resulted in unacceptable resolution, most likely caused by air bubbles. For further measurements, pre-mixed eluent was used therefore. The solution gave one very large peak at 3.6 and a smaller one at 3.04 min. Based on this result, a suitable mix of reference items was chosen (with DDT as substance with the highest log Koc and a retention time of approx. 12.2 minutes). The 50 mg/L test item solution was also used for the determination of the log Koc of the test item.
- Determination of the Dead Time: The dead time t0 was measured by using an unretained compound (sodium nitrate) which was included in the mix of the reference items.
- Determination of the Log Koc: One vial was filled with the reference item mix and one vial with the test item solution. The vials were analysed using HPLC with the program described above. Three injections were measured from the reference item mix, three injections from the test item solution and again three injections from the reference item mix. The data was saved, the retention times of the six peaks of the reference item mix and the retention time of the peaks of the test item were used for further calculations.
- Calibration log k’ vs. log Koc: The calibration function was drawn up from the six measurements of the capacity factors of the reference items. By plotting the (known) log Koc values of the reference items against the mean log capacity factors and performing linear regression, a calibration function was obtained.
- Calculation of log Koc: With the calculated capacity factors for the test item, each log Koc from the three determinations was calculated. Finally, mean and standard deviation of the log Koc were calculated. - Key result
- Type:
- log Koc
- Value:
- 1.83 dimensionless
- pH:
- 7
- Temp.:
- 25 °C
- Remarks on result:
- other: Peak 1
- Key result
- Type:
- log Koc
- Value:
- 2.36 dimensionless
- pH:
- 7
- Temp.:
- 25 °C
- Remarks on result:
- other: Peak 2
- Details on results (HPLC method):
- See 'Any other information on results and tables'.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The log Koc of FRET 11-0571 ranges from 1.83 - 2.36 (two peaks).
- Executive summary:
The adsorption potential of FRET 11 -0571 was investigated in a study according to OECD TG 121 (HPLC estimation method) and in compliance with GLP criteria (LAUS, 2016). In this study, six reference substances with known log Koc values and the test substance were analysed by HPLC. The log k’ values of reference substances were plotted against the known log Koc values. The log Koc value for the test substance was calculated by substituting its mean log k’ in the calibration curve. For the test item two peaks were found with log Koc values ranging from 1.83 to 2.36.
Reference
Table: Retention times (RT) and capacity factors of reference items
Compound |
RT mean* (min) |
RT SD (min) |
RT relative SD (%) |
k’ |
Log k’ |
Log Koc |
Sodium nitrate |
1.050 |
0.025 |
2.4 |
0.0000 |
- |
- |
Phenol |
2.638 |
0.002 |
0.1 |
1.5118 |
0.1795 |
1.32 |
Methylbenzoate |
3.043 |
0.002 |
<0.1 |
1.8969 |
0.2781 |
1.80 |
Naphthalene |
40.79 |
0.002 |
0.1 |
2.8836 |
0.4599 |
2.75 |
1,2,3-Trichlorobenzene |
4.584 |
0.002 |
<0.1 |
3.3648 |
0.5270 |
3.16 |
Phenanthrene |
6.315 |
0.006 |
0.1 |
5.0127 |
0.7001 |
4.09 |
DDT |
12.166 |
0.007 |
0.1 |
10.5828 |
1.0246 |
5.63 |
* Determined from six injections
Table: Retention times test item:
Retention times test item |
Peak 1 (min) |
Peak 2 (min) |
Measurement |
3.035 |
3.573 |
Measurement |
3.040 |
3.573 |
Measurement |
3.045 |
3.573 |
Mean |
3.040 |
3.573 |
SD |
0.005 |
0.000 |
Table: Capacity factor, log capacity factor, and log Koc of the test item:
|
Peak 1 |
Peak 2 |
||||
k’ |
Log k’ |
Log Koc |
k’ |
Log k’ |
Log Koc |
|
Measurement |
1.8897 |
0.2764 |
1.83 |
2.4016 |
0.3805 |
2.36 |
Measurement |
1.8943 |
0.2775 |
1.83 |
2.4018 |
0.3805 |
2.36 |
Measurement |
1.8994 |
0.2786 |
1.84 |
2.4019 |
0.3806 |
2.36 |
Mean |
- |
- |
1.83 |
- |
- |
2.36 |
SD |
- |
- |
0.01 |
- |
- |
0.00 |
log Koc was calculated from the capacity factor as follows: log Koc = (log k’ + 0.0774) / 0.1938
Description of key information
The log Koc ranges from 1.83 to 2.36 (two peaks). The mean value of 2.1 is used as key value for assessment.
Key value for chemical safety assessment
- Koc at 20 °C:
- 126
Additional information
[LogKoc: 2.1]
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
