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Short-term toxicity to aquatic invertebrates

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Description of key information

A study was performed to assess the acute toxicity of the test item toDaphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphniasp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.


Chemical analysis of the test preparations at 0 hours showed measured test concentrations to range from 1.40 to 51.1 mg/L. There was no significant change in the measured concentrations at 48 hours other than the exception noted below.

The 56% v/v saturated solution measured concentrations declined by 74% between 0 and48 hours. However, as there was 100% immobilization observed in lower concentrations, the 56% v/v saturated solution was not considered critical to the effect of test item exposure observed in the test and the statistical analysis. Therefore, the results are based on 0-Hour measured test concentrations only.

Exposure ofDaphnia magnato the test item gave the following results based on the 0-Hour measured test concentrations:

Time Point



95% Confidence Limits (mg/L)

No Observed Effect Concentration (NOEC) (mg/L)

Lowest Observed Effect Concentration (LOEC) (mg/L)



Not determined due to unsuitable nature of data



Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
5.5 mg/L

Additional information