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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethylhexylamine
EC Number:
203-233-8
EC Name:
2-ethylhexylamine
Cas Number:
104-75-6
Molecular formula:
C8H19N
IUPAC Name:
2-ethylhexylamine

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gassner
- Weight at study initiation: 130-200 g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 2.0, 4.0 8.0 and 20 % aqueous solution containing Traganth
Doses:
200, 400, 800, 1600 µl/kg bw = 158, 316, 632 and 1264 mg/kg bw; conversion into mg/kg is based on the density d= 0.79g/cm3 according to BASF internal data
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: several times on the day of application and daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
316 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 400 cm³/kg bw; calculated with a density of 0.79 g/cm³
Mortality:
1264 mg/kg bw dosing group: 20/20 within 1 hour
632 mg/kg bw dosing group: 20/20 within 1 hour
316 mg/kg bw dosing group: 10/20 within 1 hour
158 mg/kg bw dosing group: 0/20
Clinical signs:
200 cm³/kg bw dosing group:
strongly elevated respiration, anancasm to chew, slight salivation, slight tremor (females), fitful breathing on day 1, free of symptoms on day 2

400-800 cm³/kg bw dosing group:
strong anancasm to roll, strong twitches, dyspnoea, salivation, on day 3 without findings

1600 cm³/kg bw dosing group:
salaam convulsions, Tremor, extension convulsions, salivation, foreleg movements
Body weight:
no data
Gross pathology:
Died animals: Kidney obstructions, red-pink coloring of the stomach
Sacrificed animals: without findings

Any other information on results incl. tables

The test substance caused systemic toxicity (including mortality) in a dose dependent manner.

Mortality

 Dose (µL (kg)  Conc. (%)  No. of animals  died within  1 hour 24 hours 48 hours 7 days
 1600  20  20  20/20  20/20  20/20  20/20
 800  8  20  20/20  20/20  20/20  20/20
 400  4  20  10/20  10/20  10/20  10/20
 200  2  20  0/20  0/20  0/20  0/20

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria