Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
1996-02-22
Test type:
acute toxic class method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
potassium dibenzyl phosphate
EC Number:
616-628-9
Cas Number:
78543-37-0
Molecular formula:
C14-H14-O4-P.K
IUPAC Name:
potassium dibenzyl phosphate
Test material form:
solid
Details on test material:
Batch 97/8

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Charles River (UK) Ltd, margate, Kent
- Age at study initiation: 8 to 12 weeks of age
- Weight at study initiation: 221 to 238g
- Fasting period before study: fasting overnight the day before the dosing
- Housing: by five by sex in solid floor polypropylene cages furnished with woodflakes
- Diet (e.g. ad libitum): free access exept three or four hour after dosing (rat and mouse, expanded diet N°1, special diets services limited, witham, essex, UK)
- Water (e.g. ad libitum): fre access
- Acclimation period: At lest five days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 °C to 22°C
- Humidity (%): 48% to 63%
- Air changes (per hr): 15 changes per hour.
- Photoperiod (hrs dark / hrs light): 12h continuous light and 12hours darkness

IN-LIFE DATES: From: To: 12 may 1997 to 12 june 1997

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 200 mg/mL
- Amount of vehicle (if gavage): 10 mL

MAXIMUM DOSE VOLUME APPLIED:
10 mL/kg
Doses:
2000 mg/kg
No. of animals per sex per dose:
Range finding study: 1 male and 1 female
Main Study: 5 males and 5 females
Control animals:
no
Details on study design:
- Duration of observation period following administration:
30 minutes, 1h, 2h and 4h the first day and once daily for 14 days
- Frequency of weighing: day 0, 7 and 14
- Necropsy of survivors performed: Gross pathological examination
- Other examinations performed: clinical signs

Results and discussion

Preliminary study:
At 2000 mg/kg, there were no deaths, Hunched posture was noted during the day of dosing
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality occur
Clinical signs:
other: No sign of systemic toxicity were noted during the study
Gross pathology:
No abnormality were noted at necropsy

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The LD 50 of the dibenzyl potassium phosphate was determinated to be greater than 2000 mg/kg.
Executive summary:

The acute Oral LD50 of Dibenzyl Potassium Phosphate has been determined according to OECD 401 test guideline in compliance with GLP.

The LD50 was determinated to be greater than 2000 mg/kg.