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Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio after 96 hrs was determine to be > 100 mg/l. As no effect were observed at 100 mg/l. Based on the LC50, it can be consider that the chemical sodium naphthalene-1-sulfonate was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of sodium naphthalene-1-sulfonate to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l. Thus above result indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Toxicity to aquatic algae:

Aim of this study was to evaluate the nature of test chemical sodium naphthalene-1-sulfonate when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in OECD growth medium. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical sodium naphthalene-1-sulfonate, only 0.5 % inhibition were observed at 100 mg/l. Based on the effects on the algae it was consider that the IC50 was greater then 100 mg/l. Thus chemical consider to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

Toxicity to microorganism:

Toxicity of microorganism was evaluated for the test material Sodium 1-Naphthalenesulfonate . Water solution of test material and AS mixed before experiment and aerated for 10 min. Activated sludge having nitrifying bacteria were used obtained from a pilot activated sludge plant at Water Research Center, Swindon. In vitro; samples of actively-nitrifying sludge mixed liquor, suspended solids, SS in concentration 2000 mg/l; standard procedure for assessment of nitrification inhibition,  respiration rate of AS determined from the rate of O2 depletion measured by oxygen electrode. The nominal concentrations tested were 50 - 200 mg/l. The inhibition concentration (IC50) for the nitrifying bacteria was observed to be 926 mg/l , the (IC20) was observed to be 195 mg/l. Slightly inhibited nitrification was observed.

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical sodium naphthalene-1-sulfonate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 100mg/L ,respectively. potable water (passed through reverse osmosis system) was used and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical sodium naphthalene-1-sulfonate on Zebra Fish Danio rerio after 96 hrs was determine to be > 100 mg/l. As no effect were observed at 100 mg/l. Based on the LC50, it can be consider that the chemical sodium naphthalene-1-sulfonate was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of sodium naphthalene-1-sulfonate to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. After the exposure of test chemical with daphnia magna for 48 hrs, 0 % (No immobility) were observed at 100 mg/l. Thus The EC0 was observed at 100 mg/l. And it can be concluded that the EC50 was > 100 mg/l. Thus above result indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Toxicity to aquatic algae:

Summarized result for the toxicity of test chemical sodium naphthalene-1-sulfonate CAS No. 130 -14 -3, on the growth of algae was studied on the basis of two experimental studies are mention below:

In the first experimental study toxicity was measured. Aim of this study was to evaluate the nature of test chemical sodium naphthalene-1-sulfonate when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. Limit test performed at 100 mg/l. The solution 100 mg/l was prepared by dissolving white powder in OECD growth medium. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical sodium naphthalene-1-sulfonate, only 0.5 % inhibition were observed at 100 mg/l. Based on the effects on the algae it was consider that the IC50 was greater then 100 mg/l. Thus chemical consider to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

Similarly in the second study the effect of test substance sodium naphthalene-1-sulfonate CAS No. 130 -14 -3 was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,200 mg/L. The test concentrations were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated from equation through probit analysis was observed to be 33.47mg/L.

As the both studies occurs on two different species thus the effect of test chemical was varies. Both the studies performed according to the OECD guideline. As the other studies indicates that the chemical was nontoxic, thus overall studies consider to be nontoxic and not classified as per the CLP classification criteria.

Toxicity to microorganism:

Toxicity of microorganism was evaluated for the test material Sodium 1-Naphthalenesulfonate . Water solution of test material and AS mixed before experiment and aerated for 10 min. Activated sludge having nitrifying bacteria were used obtained from a pilot activated sludge plant at Water Research Center, Swindon. In vitro; samples of actively-nitrifying sludge mixed liquor, suspended solids, SS in concentration 2000 mg/l; standard procedure for assessment of nitrification inhibition,  respiration rate of AS determined from the rate of O2 depletion measured by oxygen electrode. The nominal concentrations tested were 50 - 200 mg/l. The inhibition concentration (IC50) for the nitrifying bacteria was observed to be 926 mg/l , the (IC20) was observed to be 195 mg/l. Slightly inhibited nitrification was observed.