Registration Dossier
Registration Dossier
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EC number: 203-597-8 | CAS number: 108-59-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dimethyl malonate
- EC Number:
- 203-597-8
- EC Name:
- Dimethyl malonate
- Cas Number:
- 108-59-8
- Molecular formula:
- C5H8O4
- IUPAC Name:
- dimethyl malonate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Preparation of inoculum: Centrifugation 10 min at 1100 x g,
the supernatant is discarded and the sludge resuspended with
mineral medium, further centrifugation for 10 min at 1100 x g
Resuspension of the activated sludge (4.9 g/l dry mass of
activated sludge)
- Initial cell concentration: 24.5 mg/l - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 9.2 mg/L
- Based on:
- DOC
- Initial conc.:
- 10.42 mg/L
- Based on:
- DOC
Results and discussion
- Preliminary study:
- INITIAL TEST SUBSTANCE CONCENTRATION: 9.2 mgDOC/l in the test flasks, 10.42 mg DOC/l in
the control flasks
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 86.5
- Sampling time:
- 7 d
- Remarks on result:
- other: Flasks with test substance and inoculum
- Parameter:
- % degradation (DOC removal)
- Value:
- 100
- Sampling time:
- 14 d
- Remarks on result:
- other: Flasks with test substance and inoculum
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 21 d
- Remarks on result:
- other: Flasks with test substance and inoculum
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 27 d
- Remarks on result:
- other: Flasks with test substance and inoculum
- Parameter:
- % degradation (DOC removal)
- Value:
- 99.5
- Sampling time:
- 28 d
- Remarks on result:
- other: Flasks with test substance and inoculum
Any other information on results incl. tables
Kinetics of biodegradation: % decrease of DOC
| day | FE1(%) | FE2(%) | FC1(%) | FC2(%) |
| 0 | 0 | 0 | 0 | 0 |
| 7 | 86 | 87 | 99 | 99 |
| 14 | 100 | 100 | 99 | 99 |
| 21 | 95 | 99 | 98 | 98 |
| 27 | 95 | 99 | 100 | 100 |
| 28 | 99 | 100 | 99 | 98 |
FE1 ans FE2: Flasks with test substance and inoculum
FC1 and FC2: Falsks control substance (sodium benzoate) and inoculum
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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