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Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Rhodium chloride (RhCl3), hydrate
EC Number:
606-630-8
Cas Number:
20765-98-4
Molecular formula:
RhCl3.3(H2O)
IUPAC Name:
Rhodium chloride (RhCl3), hydrate

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
All test concentrations analysed at 0 and 96 hours

Test solutions

Vehicle:
not specified
Details on test solutions:
Test substance was applied in geometric dilution steps.

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
Details on test organisms - source: Fischzucht Eggers Hohenwestedt
Details on test organisms - life stage of test species used: 4-7 cm at test start
Feeding: None during test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20degress C
pH:
5.82 - 8.56
Dissolved oxygen:
Not measured due to the test substance causing the oxygen meter to fail, but test solutions aerated to maintain >60% oxygen saturation
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 1000, 800, 400, 200, and 100 mg/L.
Measured concentrations at 96 hours: 991, 837, 306, 85 and 52 mg/L
Measured concentrations at 96 hours used for biological evaluations
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass vessels containing 12L test solution
- Aeration: Yes:
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality

TEST CONCENTRATIONS
- Range finding study: Yes
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
85 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
220 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
310 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
yes - no mortality in the control, oxygen concentrations did not drop in the screening test, no oxygen measurements reported for the main test due to equipment failure. Results based on measured concentrations due to loss of the test substance.
Conclusions:
The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg/L, corresponding to 86 mg Rh/L (assuming test item is rhodium trichloride,trihydrate).
Executive summary:

Kemp (1995) is a GLP compliant study following OECD guideline 203. The loss of the test substance was taken into account by reporting results based on measured concentrations. pH was outside the desired limits, oxygen measurements could not be recorded during the main test, and there are some limitations with the reporting of the methods. However, despite the limitations this study is considered valid and suitable for use as a key study for this endpoint. The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg/L, corresponding to 86 mg Rh/L (assuming test item is rhodium trichloride,trihydrate).