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EC number: 255-707-9 | CAS number: 42204-14-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Diammonium sodium hexakis(nitrito-N)rhodate
- EC Number:
- 264-713-0
- EC Name:
- Diammonium sodium hexakis(nitrito-N)rhodate
- Cas Number:
- 64164-17-6
- Molecular formula:
- H4N.1/2N6O12Rh.1/2Na
- IUPAC Name:
- Diammonium sodium hexakis(nitro-N)rhodate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples taken at test start and end from all test concentrations and control.
- Sampling method: Due to turbidity, test media in the two highest test concentrations was filtered before use in the algal test and sampling
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was dissolved in growth medium and diluted with growth medium to obtain the required test concentrations under sterile conditions. The highest test concentration was prepared by adding the appropriate amount of test substance to the dilution water and vigorously stirring for 1 hour at 20°C. An aliquot of this test solution was then diluted further to prepare the other test concentrations.
- Controls: Dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None used
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches, Institut of the University at Göttingen, Albrecht von Haller, Institut, Untere Klarspüle 2, D-37073 Göttingen, Catalog No 61.81 SAG
- Method of cultivation: Prior to testing a pre-culture was established in standard OECD growth medium to obtain exponentially-growing algae for the test. The culture duration of the pre-cultures was 3 days.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.0 -22.1°C
- pH:
- pH of the controls: 8.03 at test start and 7.81 at test end (mean)
pH in test cultures: 7.3 - 8.07 at test start and 7.17 - 7.95 at test end (mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 mL conical glass flasks covered with air-permeable silicone sponge caps. Test vessels were cleaned using a dishwasher and additionally rinsed six times with purified water. Test vessels and caps were sterilised by autoclaving prior to use.
- Renewal rate of test solution (frequency/flow rate): Static
- Initial cells density: 10,000 cells/mL
- Control end cells density: 1,832,880 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes – OECD 201 growth medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: No
- Light intensity and quality: 92.65 – 98.29 µMol s-1 m-2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Determination of cell concentrations: measured at 24, 48 and 72 hours using an electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: Concentrations based on a previous definitive test (Wenzel 2015a)
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 4.23 - 4.78 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.782
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 0.705 - 0.862 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.313 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 0.273 - 0.356 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.301 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.522
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 95% CL 0.477 - 0.568 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.131 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 95% CL 0.109 - 0.155 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- < 0.301 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.301 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.656 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 0.616 - 0.696
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.046 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 0.040 - 0.052
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.044 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.076 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 95% CL 0.069 - 0.083
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- < 0.044 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.044 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Basis for effect:
- other: Yield
- Results with reference substance (positive control):
- ErC50: 2.62 mg/L(August 2015). Result is in good agreement with an international ring test
- Reported statistics and error estimates:
- EC50, EC20 and EC10, and the respective 95% confidence intervals, were calculated using Probit-analysis assuming log-normal distribution of the values. The NOEC values for growth rate were determined by the Williams Multiple Sequential t-test and the NOEC values for yield were calculated by the Mediantest (2-by-2-table) with Bonferroni correction, both using the computer program ToxRat Professional.
Any other information on results incl. tables
Table 1: Effective concentrations of Diammonium sodium hexakis (nitrito-N) rhodate for the exposure of Pseudokirchneriella subcapitata for 72 hours | ||||
Parameter | EC50 [mg/L] | EC20 [mg/L] | EC10 [mg/L] | NOEC [mg/L] |
Measured initial concentrations [mg test item/L] | ||||
Growth rate (r) Value | 4.5 | 0.782 | 0.313 | <0.301 |
Yield (y) Value | 0.522 | 0.131 | <0.301 | <0.301 |
Table 2: Effective concentrations in terms of rhodium concentrations for the exposure of Pseudokirchneriella subcapitata for 72 hours | ||||
Parameter | EC50 [mg/L] | EC20 [mg/L] | EC10 [mg/L] | NOEC [mg/L] |
Measured initial concentrations [mg test item/L] | ||||
Growth rate (r) Value | 0.656 | 0.114 | 0.046 | <0.044 |
Yield (y) Value | 0.076 | 0.019 | <0.044 | <0.044 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The cell no. in controls inc. by a factor of 183. The mean of replicate coefficients of variations in section-by-section growth rate of controls - 14.2%. The coefficient of variation of average specific growth rate at test end in replicate control - 2.4%.
- Conclusions:
- The 72-hour EC50, EC10 and NOEC were found to be 4.5, 0.313 and < 0.301 mg test item L-1, respectively, for growth rate (corresponding to 0.656, 0.046 and <0.044 mg Rh L-1).
- Executive summary:
The effect of diammonium sodium hexakis (nitrito-N) rhodate on the growth rate and yield of Pseudokirchneriella subcapitata was investigated according to OECD guideline 201 and EEC method C.3 (Wenzel 2015). Algae were placed in test medium containing the test item at 7 nominal concentrations ranging from 0.20 to 214.7 mg test item L-1, corresponding to nominal rhodium concentrations of 0.029 to 31.3 mg Rh L-1. The test was conducted under static conditions for 72 hours. The test solutions were analysed for rhodium using ICP-OES and test results reported based on measured initial concentrations.
The 72-hour EC50, EC10 and NOEC were found to be 4.5, 0.313 and < 0.301 mg test item L-1 (0.656, 0.046 and <0.044 mg Rh L-1), respectively, for growth rate. For yield, the EC50, EC10 and NOEC were 0.522, <0.301 and <0.301 mg test item L-1 (0.076, <0.044 and <0.044 mg Rh L-1), respectively.
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