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Description of key information

Acute oral toxicity

LD50 was estimated to be 2630 mg/kg bw when rats were orally exposed with N, N, N-triethylethanaminium chloride (56-34-8)

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer-review journal
Qualifier:
equivalent or similar to
Guideline:
other: As mention below
Principles of method if other than guideline:
Acute oral toxicity study of N, N, N-triethylethanaminium chloride was performed in Rat
GLP compliance:
not specified
Test type:
fixed dose procedure
Limit test:
yes
Specific details on test material used for the study:
Name of test material (as cited in study report):Tetraethylammonium chloride
- Molecular formula :C8H20N.CI
- Molecular weight :165.706 g/mole
- Substance type:organic
- Physical state:Crystalline powder
-Purity:No data available
- Impurities (identity and concentrations):No data available
Species:
rat
Strain:
other: Albino
Sex:
not specified
Details on test animals and environmental conditions:
No data available
Route of administration:
oral: unspecified
Vehicle:
water
Details on oral exposure:
Details on exposure
VEHICLE
- Concentration in vehicle: 40.6% test substance dissolved in distilled water
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: Readily dissolved in water
- Lot/batch no. (if required): No data available
- Purity: No data available

MAXIMUM DOSE VOLUME APPLIED: No data available
DOSAGE PREPARATION (if unusual): No data available
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: No data available
Doses:
2630mg/kg
No. of animals per sex per dose:
155
Control animals:
no
Details on study design:
No data available
Statistics:
No data available
Preliminary study:
No data available
Sex:
not specified
Dose descriptor:
LD50
Effect level:
2 630 mg/kg bw
Based on:
test mat.
Remarks on result:
other: Mortality observed
Mortality:
Mortality observed with 10-30 min due to respiratory failure
Clinical signs:
Tremors ,incoordination ,flaccid prostration
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available
Interpretation of results:
other: Not classified
Conclusions:
LD50 was consiidered to be 2630mg/kg body weight .When albino rats were treated with N, N, N-triethylethanaminium chloride (56-34-8)
Executive summary:

Acute oral toxicity study was done in albino rat using test material N, N, N-triethylethanaminium chloride (56-34-8).155 animals were used. 40.6% test substance dissolved in distilled water to get required dose concentration. Maximum tolerated dose i.e.95-100 % animal survived was determined to be 750.0mg/kg .Clinical signslikeTremors, incoordination, flaccid prostrationwere observed. Mortality observed with 10-30 min due to respiratory failure at dose 2630mg/kg/bw .Hence LD50 was determined2630mg/kg body weight. When albino rats were treated with N, N, N-triethylethanaminium chloride (56-34-8)

Endpoint conclusion
Endpoint conclusion:
adverse effect observed
Dose descriptor:
LD50
Value:
2 630 mg/kg bw
Quality of whole database:
Data is K2 obtain from peer- reviewed journal

Acute toxicity: via inhalation route

Endpoint conclusion
Endpoint conclusion:
no study available

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

In different studies N, N, N-triethylethanaminium chloride (56-34-8)has been investigated for acute oral toxicity to a greater or lesser extent. Often are the studies based on in vivo experiments and estimated data in rodents, i.e. most commonly in rats and mouse forN, N, N-triethylethanaminium chloride (56-34-8).

In experimental study for Tetraethylammonium chloride (CAS no 56-34-8) by Gruhzitet. al.(1948), acute oral toxicity was evaluated on albino rats by using Tetraethyl ammonium chloride in the concentration of 40.6 per cent commercial aqueous stock solution suitably diluted with distilled water. 95 to 100 per cent of animals survive at 750.0 mg/kg. The dose was considered to be maximum tolerated dose and 50 % mortality was observed at 2630.0 mg/kg in treated rats. Therefore, LD50 of Tetraethyl ammonium chloride was considered to be 2630.0 mg/kg in rats.

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated forN,N,N-triethylethanaminium chloride (56-34-8),LD50 was estimated to be 2862mg/kg bw, when male and female Sprague-Dawley ratswereexposed withN,N,N-triethylethanaminium chloride (56-34-8)orally.

Also it is further supported by experimental study given by U.S. National Library of Medicine (ChemIDplus A TOXNET Database, 2017) on structurally for read across Tetrylammonium bromide (CAS no 71-91-0), acute oral toxicity was evaluated in mice by using Tetraethyl ammonium chloride at 2000 mg/kg. 50 % mortality was not observed at 2000 mg/kg. Therefore, LD50 of Tetrylammonium bromide (TEA) was considered to be >2000 mg/kg in mouse.

 

Also it is further supported by experimental study given by U.S. National Library of Medicine (ChemIDplus A TOXNET Database, 2017) on structurally similar read across substanceTetraethyl ammonium(66-40-0),mice were treated withTetraethyl ammonium(66-40-0))orally. No mortality was observed in treated rats at 2000 mg/kg bw. Therefore, LD50 was considered to be 2000mg/kg bw when rats were treated withTetraethyl ammonium(66-40-0), orally.  

 

Thus, based on the above studies onN, N, N-triethylethanaminium chloride (56-34-8)and its read aross substancesTetrylammonium bromide (CAS no 71-91-0) andTetraethyl ammonium(66-40-0). It can be concluded that LD50 value is 2630.0mg/kg bw by oral route . Thus, comparing this value with the criteria of CLP regulationN, N, N-triethylethanaminium chloride (56-34-8)can be “not classified” of acute toxicity.

 

Justification for classification or non-classification

Thus, comparing this value with the criteria of CLP regulationN, N, N-triethylethanaminium chloride (56-34-8)can be “not classified” of acute toxicity.