Ethyldimethylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions, Non-GLP

Qualifier:

according to guideline

Guideline:

other: The study was performed according to German national standard DIN 38 412, part L15 (status: Jan 1979.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldenorfe
- Strain: Leuciscus idus L. (golden variety)
- Source: : Fish farm Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 6.4 cm
- Weight at study initiation (mean and range, SD): 2.3 g

ACCLIMATION
- Acclimation conditions:
Culture conditions: fish were kept in oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature:20 ±1 °C
pH: 7-8
- Type and amount of food: Fukosalm- Anschlussfutter 1
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): no mortality during the last 6 weeks of housing.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

total hardness: 2.5 mmol/L

Test temperature:

20 ± 1 °C

pH:

8 ±0.1

Dissolved oxygen:

> 8 mg/L

Nominal and measured concentrations:

Nominal concentrations: 6.8, 10, 14.7, 21.5, 31.6, 46.4, 68.1, 100 mg/L. Additionally: 100 mg/L pH-adjusted

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration wiht oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

Reference substance (positive control):

yes

Remarks:

Chloracetamid

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

31.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

31.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

38.29 other: mg/L (geometric mean)

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Neutralized

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: tumbling, lateral position

Results with reference substance (positive control):

- Results with reference substance valid? yes
- LC50: 26 mg/L

Sublethal observations / clinical signs:

Cumulative mortality

Nominal conc.(mg/l)	Number of fish	Mortality (cumulated) after
		1 h	4 h	24 h	48 h	72 h	96 h
0	10	0	0	0	0	0	0
6.8	10	0	0	0	0	0	0
10.0	10	0	0	0	0	0	0
14.7	10	0	0	0	0	0	0
21.5	10	0	0	0	0	0	0
31.6	10	0	0	0	0	0	0
46.4	10	1	10	10	10	10	10
68.1	10	10	10	10	10	10	10
100	10	10	10	10	10	10	10

Symptoms

Nominal conc.(mg/l)	Number of fish	Symptoms after
		4 h
0	10
6.8	10
10.0	10
14.7	10
21.5	10
31.6	10
46.4	10	Tumbling, lateral position
68.1	10
100	10

pH values

Nominal conc.(mg/l)	pH values after
	1 h	24 h	48 h	72 h	96 h
0	7.4	7.6	7.6	7.6	7.6
6.8	7.9	7.8	7.7	7.7	7.8
10.0	8.6	7.6	7.6	7.6	7.7
14.7	8.8	7.8	7.7	7.8	7.8
21.5	9.3	7.6	7.5	7.6	7.6
31.6	9.5	7.8	7.7	7.7	7.7
46.4	9.8	9.3
68.1	10.1	9.3
100	10.6	9.9

Oxygen content

Nominal conc.(mg/l)	Oxygen concentrations [mg/L] after
	1 h	24 h	48 h	72 h	96 h
0	8.8	8.5	8.7	8.5	8.4
6.8	9.0	9.0	9.0	8.6	8.6
10.0	8.4	8.5	8.6	8.4	8.4
14.7	8.9	8.9	8.9	8.7	8.8
21.5	8.4	8.0	8.4	8.2	8.3
31.6	8.7	8.4	8.4	8.4	8.3
46.4	8.5	9.1
68.1	9.1	9.2
100	9.1	9.2

Observed effects may be due to a pH-shift at higher test concentrations. After neutralization (100 mg/L) no effects were observed.

Validity criteria fulfilled:

not specified

Conclusions:

Ethyldimethylamine is considered harmful to fish as a worst-case.

Executive summary:

Ethyldimethylamine acute toxicity to Leuscisus idus has been investigated according to German industrial standard test guideline DIN 38412 part 15. Ethyldimethylamine acute toxicity (LC50 -96h) ranges from 38.29 to >100 mg/L depending on the neutralization state.

Description of key information

One study has been identified as relevant for characterizing ethyldimethylamine toxicity to fish. In the framework of this study carried out according to National German guidelines, the toxicity determined is to be 38.29 mg/L (LC50 -96h, pH non-adjusted). No mortality is observed up to 100 mg/L when pH was adjusted.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

38.29 mg/L