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EC number: 264-780-6 | CAS number: 64338-16-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline / GLP Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2,4,4-tetramethyl-7-oxa-3,20-diazadispiro[5.1.11.2]-henicosan-21-one
- EC Number:
- 264-780-6
- EC Name:
- 2,2,4,4-tetramethyl-7-oxa-3,20-diazadispiro[5.1.11.2]-henicosan-21-one
- Cas Number:
- 64338-16-5
- Molecular formula:
- C22H40N2O2
- IUPAC Name:
- 2,2,4,4-tetramethyl-7-oxa-3,20-diazadispiro[5.1.11⁸.2⁶]henicosan-21-one
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Non adapted activated sludge from the sewage municipal plant at D-31137 Hildesheim. The activated sludge was washed twice with autoclaved tap water and maintained in an aerobic condition by aeration for 4 hours and then homogenized with a mixer for 2 min. Thereafter the sludge was filtered with folded filter and aerated with C02-free air for 5 days. 25 mL/L were used to initiate inoculation.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 6 d
- Remarks on result:
- other: test item, 1streplicate 15 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 14 d
- Remarks on result:
- other: test item, 1streplicate 15 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 12
- Sampling time:
- 21 d
- Remarks on result:
- other: test item, 1streplicate 15 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 35
- Sampling time:
- 28 d
- Remarks on result:
- other: test item, 1streplicate 15 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0 - 1
- Sampling time:
- 28 d
- Remarks on result:
- other: test item, 2ndreplicate 15 mg/L
- Details on results:
- The 10 % level (beginning of biodegradation) was only reached by one replicate after a long lasting adaptation period of 20 days, the degradation came to a maximum of 35 % after 28 days. The biodegradation of the other replicate remained at a level of 1 % after 28 days.
In the toxicity control 30 % biodegradation occurred within 14 days and came to a maximum of 47 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
BOD5 / COD results
- Results with reference substance:
- To check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 8 days. In the toxicity control containing both test and reference item a biodegradation rate of 30 % occurred within 14 days and came to a maximum of 47 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
Any other information on results incl. tables
Biodegradation [%] study day [d] |
||||
6 | 14 | 21 | 28 | |
test item, 1streplicate 15 mg/L |
1 |
0 |
12 |
35 |
test item, 2ndreplicate 15 mg/L |
0 | 0 | 0 | 1 |
functional control 35 mg/L |
50 |
74 |
83 |
95 |
toxicity control 15 mg/L test item + 35 mg/L reference item |
15 |
30 |
34 |
47 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
- Executive summary:
The ready biodegradability was determined with a non adapted activated sludge for the test item Hostavin N20 P according to OECD 301 B / C02 evolution test. The test item was tested with a concentration of 15 mg/L in duplicates, corresponding to a carbon content (TOC) of 10.9 mgC/L in the test vessels. The biodegradation of the test item was followed by titrimetric analyses of the quantity of C02 produced by the respiration of bacteria. The degradation was finished on day 28 by acidification, the last titration was made on day 29, after the soluble C02 was turned out over a period of 24 h. The percentage C02 production was calculated in relation to the theoretical CO2 (ThC02) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 8 days. In the toxicity control containing both test and reference item a biodegradation rate of 30 % occurred within 14 days and came to a maximum of 47 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The 10 % level (beginning of biodegradation) was only reached by one replicate after a long lasting adaptation period of 20 days, the degradation came to a maximum of 35 % after 28 days. The biodegradation of the other replicate remained at a level of 1 % after 28 days. The validity criteria according to the guideline are fulfilled.
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