Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
6th January to 27 Februaru 2003
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study was conducted in a GLP compliant laboratory according to recognised regulatory guidelines
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: None
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Due to the low aqueous solubility and complex nature of the test material for the purposes of the definitive test the test material was prepared as a Water Accommodated Fraction (WAF).
Amounts of test material (20, 36, 64 and 112 mg) were each separately weighed onto a microscope slide which was suspended below the surface of 20 litres of reconstituted water to give the 1.0, 1.8, 3.2 and 5.6 mg/lloading rates respectively. Further amounts of test material (25, 45, 80, 140 and 250 mg) were each separately weighed onto a microscope slide which was suspended below the surface of 2.5 litres of reconstituted water to give the 10, 18, 32, 56 and 100 mg/lloading rates respectively. The test material was weighed onto microscope slides to ensure accuracy of weighing due to the small amounts used and the highly viscous nature of the test material. After addition ofthe test material, the reconstituted water was stirred for 23 hours by magnetic stirrer such that a vortex depth of approximately 25% of the overall height of the water column was achieved. The stirring was stopped after 23 hours and the mixtures allowed to stand for 1 hour prior to removing the aqueous phase or WAF by mid-depth siphoning to give the 1.0, 1.8,3.2,5.6, 10, 18,32,56 and 100 mg/lloading rate WAFs.
Information provided by the Sponsor indicated that the test material may undergo photo-oxidation when exposed to natural or artificial daylight. Therefore, all media preparation was conducted under laboratory safety lighting (low intensity non-actinic light source used to prevent photodegradation of light sensitive materials). The remainder of media preparation (stirring and standing) and the exposure phase ofthe test were performed shielded from the light.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name:
- Strain: N/a
- Source:in-house laboratory cultures
- Age at study initiation (mean and range, SD): 1st instar
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding: parthenogenesis
- Feeding during test Yes
- Food type: suspension of algae (Chlorella sp.)
- Amount: Not specified
- Frequency: daily

- Acclimation period: N/a
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: suspension of algae (Chlorella sp.)
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): No

Test type:
Water media type:
250 mg/L as CaCO3
Test temperature:
7.9 - 8.0
Dissolved oxygen:
8.0 - 8.2 mgO2/L (90 - 92% ASV)
Nominal and measured concentrations:
NOMINAL CONCENTRATIONS: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
Measured concentrations: The test material was not detected at the initial time point and after 48 hours in the test medium in light and dark conditions.
Key result
48 h
Dose descriptor:
Effect conc.:
15 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 95% CL
Key result
48 h
Dose descriptor:
Effect conc.:
5.6 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Validity criteria fulfilled:
The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL·50 value of 15 mg/l loading rate WAF with 95% confidence limits of 13 - 18 mg/l loading rate WAF. The No Observed Effect Loading rate at 48 hours was 5.6 mg/ lloading rate WAF.

Description of key information

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
15 mg/L

Additional information

The EL50 value refers to nominal loading level resulting from water accomodated fraction (WAF). Water solubility of the substance is < 0.1 mg/l.