magnesium sodium fluoride silicate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011-07-11 to 2011-08-25

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004-04-13

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Topy Industries / 91108
- Expiration date of the lot/batch: 2014-11-20

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: soluble

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 100mg/L

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Solution
- Controls: K2Cr2O7
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): visual inspection

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Cat# 142330
- Source: Carolina Biological Supply Company (Burlington, NC)
- Age of parental stock (mean and range, SD): < 24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20.1 - 20.5 °C

pH:

7.30 to 7.59

Dissolved oxygen:

Test initiation: 6.43 mg/L
after 24 h: 5.4 - 5.7 mg/L
and of test (48 h): 4.7 - 4.8 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: test tube
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 10 ml fill volume
- Aeration: no
- No. of organisms per vessel: 5
- replicates: 5 for solvent control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural spring water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h / 12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

VEHICLE CONTROL PERFORMED: yes

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: immobilsation
- Limit test: no
- Dose-response test: yes

Validity criteria fulfilled:

yes

Conclusions:

The substance magnesium sodium fluoride silicate did not exhibit aquatic toxicity to Daphnia magna in a test according to OECD 202.

Executive summary:

The short-term toxicity study on daphnia for magnesium sodium fluoride silicate EC# 442-650-1, CAS# 56450-90-9 was determined in a limit test according to OECD 202. No toxicity occured in the limit test with 100 mg/L.

The substance magnesium sodium fluoride silicate did not exhibit aquatic toxicity to Daphnia magna in a test according to OECD 202.

Description of key information

Th substance magnesium sodium fluoride silicate did not exhibit aquatic toxicity to Daphnia magna in a test according to OECD 202.