1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2008

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)

Version / remarks:

1996

GLP compliance:

not specified

Details on sampling:

Samples from both test solutions and test organisms were taken daily from the 26th day to the last day during the 28-day exposure test period. Samples were prepared by extracting the lipid component from the test fish.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Nominal and measured concentrations:

Nominal concentration of 20 mg/L (mean measured concentration <0.023 mg/L)

Reference substance (positive control):

not specified

Conc. / dose:

<= 0.02 mg/L

Type:

BCF

Value:

< 100 dimensionless

Remarks on result:

other: limited details are available

Measured concentration of Disperse Blue 77, fish lipid content and BCF calculation

Treatments (20 mg/L)		26^thday	27^thday	28^thday
	Measured concentration of the test substance in extracted solutions (mg/L)	< 0.02	< 0.02	< 0.02
	Content of the test substance in the fish lipids (mg)	< 0.002	< 0.002	< 0.002
	Fish total weight (g)	3.07	3.47	3.11
	Concentration of the test substance in the fish Cf(mg/kg)	< 0.65	< 0.58	< 0.64
	Measured concentration of the test substance in the water Cw(mg/L)	< 0.023	< 0.023	< 0.023
	Fish lipid content (%)	1.37	1.95	1.47
	BCF	< 100	< 100	< 100
	Average BCF	< 100

Validity criteria fulfilled:

not specified

Conclusions:

The BCF for Disperse Blue 077 was determined to be <100.

Executive summary:

The bioconcentration of Disperse Blue 077 in Zebra fish (Brachydanio rerio) was determined in a 28-day semi-static test with a test medium renewal every two days. An exposure test at a nominal concentration of 20 mg/L (mean measured concentration <0.023 mg/L) was performed in accordance with the result of the fish acute toxicity test to check the bioconcentration potential of the test substance. Samples from both test solutions and test organisms were taken daily from the 26th day to the last day during the 28-day exposure test period. Samples were prepared by extracting the lipid component from the test fish. Based on the findings of the study, the BCF for Disperse Blue 077 was determined to be <100. Lack of detection in fish extracts (< 0.02 mg/L) suggests a limited solubility in lipids and/or limited potential to partition into fish tissue from aqueous systems - most likely both.

Description of key information

The BCF for Disperse Blue 077 was determined to be <100. The bioconcentration of Disperse Blue 077 in Zebra fish (Brachydanio rerio) was determined in a 28-day semi-static test with a test medium renewal every two days. An exposure test at a nominal concentration of 20 mg/L (mean measured concentration <0.023 mg/L) was performed. Based on the findings of the study, the BCF for Disperse Blue 077 was determined to be <100. Lack of detection in fish extracts (< 0.02 mg/L) suggests a limited solubility in lipids and/or limited potential to partition into fish tissue from aqueous systems - most likely both.

Key value for chemical safety assessment

BCF (aquatic species):

100 dimensionless