Cinnamyl propionate

Endpoint summary

Description of key information

Skin Irritation:

No irritation was observed after 48-hours in human volunteers. Hence, Cinnamyl propionate can be considered to be not irritating to human skin.

Eye Irritation:

The ocular irritation potential of Cinnamyl propionate was estimated using OECD QSAR toolbox v3.3 with logPow as the primary descriptor.

Cinnamyl propionate was estimated to be not irritating to the eyes of New Zealand White rabbits.

 Based on the estimated results, Cinnamyl propionate can be considered to be not irritating to eyes and can be classified under the category “Not Classified” as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from peer reviewed journals

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To assess the dermal irritation potential of cinnamyl propionate in humans

GLP compliance:

not specified

Specific details on test material used for the study:

Name of the test chemical:3-phenylprop-2-en-1-yl propionate, Cinnamyl propionate
Molecular Formula: C12H14O2
Molecular Weight: 190.241 g/mol
InChI: 1S/C12H14O2/c1-2-12(13)14-10-6-9-11-7-4-3-5-8-11/h3-9H,2,10H2,1H3/b9-6+
Substance Type: Organic
Physical State: Liquid

Species:

other: humans

Strain:

not specified

Details on test animals or test system and environmental conditions:

sex: male

Type of coverage:

occlusive

Preparation of test site:

not specified

Vehicle:

other: Petrolatum

Controls:

not specified

Amount / concentration applied:

4% in petrolatum

Duration of treatment / exposure:

48 hours

Observation period:

48 hours

Number of animals:

5 male volunteers

Details on study design:

TEST SITE
- Area of exposure: on the backs

Other effects / acceptance of results:

no data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

48 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No irritation was observed

Interpretation of results:

other: not irritating

Conclusions:

No irritation was observed after 48-hours in human volunteers. Hence, Cinnamyl propionate can be considered to be not irritating to human skin.

Executive summary:

A skin irritation test was conducted on human volunteers to assess the skin irritancy ofCinnamyl propionate.

4% cinnamyl propionate in petrolatum was applied on the backs of 5 healthy, male volunteers in a 48-h closed patch test. Human volunteers were observed for signs of irritation.

No irritation was observed after 48-hours in human volunteers. Hence, Cinnamyl propionate can be considered to be not irritating to human skin.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

data is from OECD QSAR toolbox v3.3 and the QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: Estimated data

Principles of method if other than guideline:

Prediction was done using OECD QSAR toolbox v3.3

GLP compliance:

not specified

Specific details on test material used for the study:

Name of the test chemical: 3-phenylprop-2-en-1-yl propionate, Cinnamyl propionate
Molecular Formula: C12H14O2
Molecular Weight: 190.241 g/mol
InChI: 1S/C12H14O2/c1-2-12(13)14-10-6-9-11-7-4-3-5-8-11/h3-9H,2,10H2,1H3/b9-6+
Substance Type: Organic
Physical State: Liquid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

no data available

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

0.1 ml

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

1,24,48 and 72 hours

Duration of post- treatment incubation (in vitro):

no data available

Number of animals or in vitro replicates:

6

Details on study design:

no data available

Other effects / acceptance of results:

no data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

no irritation observed

Estimation method: Takes mode value from the 7 nearest neighbours
Domain  logical expression:Result: In Domain

(((((((((((((((("a" or "b" or "c" or "d" or "e") and("f" and(not "g")) ) and("h" and(not "i")) ) and("j" and(not "k")) ) and("l" and(not "m")) ) and("n" and(not "o")) ) and("p" and(not "q")) ) and "r") and "s") and("t" and(not "u")) ) and("v" and(not "w")) ) and "x") and("y" and(not "z")) ) and("aa" and(not "ab")) ) and("ac" and(not "ad")) ) and("ae" and "af") )

Domain logical expression index: "a"

Referential boundary:The target chemical should be classified as Esters (Acute toxicity) by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary:The target chemical should be classified as SN2 AND SN2 >> SN2 Reaction at a sp3 carbon atom AND SN2 >> SN2 Reaction at a sp3 carbon atom >> Activated alkyl esters and thioesters  by Protein binding by OASIS v1.3

Domain logical expression index: "c"

Referential boundary:The target chemical should be classified as SN2 AND SN2 >> SN2 reaction at sp3 carbon atom AND SN2 >> SN2 reaction at sp3 carbon atom >> Allyl acetates and related chemicals by Protein binding by OECD

Domain logical expression index: "d"

Referential boundary:The target chemical should be classified as Class 3 (unspecific reactivity) by Acute aquatic toxicity classification by Verhaar (Modified)

Domain logical expression index: "e"

Referential boundary:The target chemical should be classified as Esters AND Vinyl/Allyl Esters by Aquatic toxicity classification by ECOSAR

Domain logical expression index: "f"

Referential boundary:The target chemical should be classified as No alert found by DNA binding by OECD

Domain logical expression index: "g"

Referential boundary:The target chemical should be classified as Acylation OR Acylation >> Direct Addition of an Acyl Halide OR Acylation >> Direct Addition of an Acyl Halide >> Acyl halide OR Acylation >> Direct Addition of an Acyl Halide >> Alkyl carbamyl halides OR Acylation >> Isocyanates and Isothiocyanates OR Acylation >> Isocyanates and Isothiocyanates >> Isocyanates OR Acylation >> Isocyanates and Isothiocyanates >> Isothiocyanates OR Acylation >> P450 Mediated Activation to Isocyanates or Isothiocyanates OR Acylation >> P450 Mediated Activation to Isocyanates or Isothiocyanates >> Benzylamines-Acylation OR Acylation >> P450 Mediated Activation to Isocyanates or Isothiocyanates >> Formamides OR Michael addition OR Michael addition >> P450 Mediated Activation of Heterocyclic Ring Systems OR Michael addition >> P450 Mediated Activation of Heterocyclic Ring Systems >> Furans OR Michael addition >> P450 Mediated Activation of Heterocyclic Ring Systems >> Thiophenes-Michael addition OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Alkyl phenols OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Arenes OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Hydroquinones OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Methylenedioxyphenyl OR Michael addition >> Polarised Alkenes-Michael addition OR Michael addition >> Polarised Alkenes-Michael addition >> Alpha, beta- unsaturated aldehydes OR Michael addition >> Polarised Alkenes-Michael addition >> Alpha, beta- unsaturated amides OR Michael addition >> Polarised Alkenes-Michael addition >> Alpha, beta- unsaturated esters OR Michael addition >> Polarised Alkenes-Michael addition >> Alpha, beta- unsaturated ketones OR Schiff base formers OR Schiff base formers >> Direct Acting Schiff Base Formers OR Schiff base formers >> Direct Acting Schiff Base Formers >> Mono aldehydes OR SN1 OR SN1 >> Carbenium Ion Formation OR SN1 >> Carbenium Ion Formation >> Alpha halo ethers (including alpha halo thioethers) OR SN1 >> Iminium Ion Formation OR SN1 >> Iminium Ion Formation >> Aliphatic tertiary amines OR SN1 >> Nitrenium Ion formation OR SN1 >> Nitrenium Ion formation >> Aromatic azo OR SN1 >> Nitrenium Ion formation >> Aromatic nitro OR SN1 >> Nitrenium Ion formation >> Primary (unsaturated) heterocyclic amine OR SN1 >> Nitrenium Ion formation >> Primary aromatic amine OR SN1 >> Nitrenium Ion formation >> Secondary aromatic amine OR SN1 >> Nitrenium Ion formation >> Tertiary (unsaturated) heterocyclic amine  OR SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine OR SN1 >> Nitrenium Ion formation >> Unsaturated heterocyclic azo OR SN2 OR SN2 >> Direct Acting Epoxides and related OR SN2 >> Direct Acting Epoxides and related >> Epoxides OR SN2 >> Episulfonium Ion Formation OR SN2 >> Episulfonium Ion Formation >> Mustards OR SN2 >> P450 Mediated Epoxidation OR SN2 >> P450 Mediated Epoxidation >> Coumarins OR SN2 >> P450 Mediated Epoxidation >> Thiophenes-SN2 OR SN2 >> SN2 at an sp3 Carbon atom OR SN2 >> SN2 at an sp3 Carbon atom >> Aliphatic halides OR SN2 >> SN2 at an sp3 Carbon atom >> Phosphonic esters OR SN2 >> SN2 at an sp3 Carbon atom >> Sulfates OR SN2 >> SN2 at an sp3 Carbon atom >> Sulfonates by DNA binding by OECD

Domain logical expression index: "h"

Referential boundary:The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "i"

Referential boundary:The target chemical should be classified as Moderate binder, NH2 group OR Moderate binder, OH grooup OR Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non binder, non cyclic structure OR Strong binder, OH group OR Weak binder, NH2 group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "j"

Referential boundary:The target chemical should be classified as No alert found by Protein binding alerts for Chromosomal aberration by OASIS v1.1

Domain logical expression index: "k"

Referential boundary:The target chemical should be classified as Ac-SN2 OR Ac-SN2 >> Acylation involving an activated (glucuronidated) carboxamide group OR Ac-SN2 >> Acylation involving an activated (glucuronidated) carboxamide group >> Carboxylic Acid Amines OR Ac-SN2 >> Acylation involving an activated (glucuronidated) ester group OR Ac-SN2 >> Acylation involving an activated (glucuronidated) ester group >> Arenecarboxylic Acid Esters OR Ac-SN2 >> Acylation involving an activated (glucuronidated) sufonamide group OR Ac-SN2 >> Acylation involving an activated (glucuronidated) sufonamide group >> Arenesulphonamides OR Ac-SN2 >> Direct acylation involving a leaving group OR Ac-SN2 >> Direct acylation involving a leaving group >> Carboxylic Acid Amines OR Ac-SN2 >> Ring opening acylation reaction OR Ac-SN2 >> Ring opening acylation reaction >> Carboxylic acid Anhydrides OR AN2 OR AN2 >> Michael addition to activated double bonds OR AN2 >> Michael addition to activated double bonds >> alpha, beta - Unsaturated Carbonyls and Related Compounds OR AN2 >> Michael addition to activated double bonds in heterocyclic ring systems OR AN2 >> Michael addition to activated double bonds in heterocyclic ring systems >> Pyrazolone and Pyrazolidine Derivatives OR AN2 >> Michael-type addition to quinoid structures OR AN2 >> Michael-type addition to quinoid structures >> Carboxylic Acid Amines OR AN2 >> Nucleophilic addition at polarized N-functional double bond OR AN2 >> Nucleophilic addition at polarized N-functional double bond >> Arenesulphonamides OR AN2 >> Shiff base formation with carbonyl compounds OR AN2 >> Shiff base formation with carbonyl compounds >> Pyrazolone and Pyrazolidine Derivatives OR SN2 OR SN2 >> Alkylation, nucleophilic subsitution at sp3-Carbon atom OR SN2 >> Alkylation, nucleophilic subsitution at sp3-Carbon atom >> Alpha-Activated Haloalkanes by Protein binding alerts for Chromosomal aberration by OASIS v1.1

Domain logical expression index: "l"

Referential boundary:The target chemical should be classified as Inclusion rules not met by Eye irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "m"

Referential boundary:The target chemical should be classified as Aliphatic esters of chloro formic acid OR Aliphatic monoalcohols OR Organic sulphonic salts OR Subststituted pyrazoles OR Thiazoles and thiazolidines by Eye irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "n"

Referential boundary:The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "o"

Referential boundary:The target chemical should be classified as (!Undefined)Group CN Lipid Solubility < 0.4 g/kg OR (!Undefined)Group CNHal Lipid Solubility < 400 g/kg OR Group All Aqueous Solubility < 0.00002 g/L OR Group All log Kow < -3.1 OR Group All Melting Point > 200 C OR Group C Aqueous Solubility < 0.0001 g/L OR Group C Aqueous Solubility < 0.0005 g/L OR Group C Melting Point > 55 C OR Group C Molecular Weight > 380 g/mol OR Group CHal log Kow > 4.5 OR Group CHal Melting Point > 65 C OR Group CHal Molecular Weight > 280 g/mol OR Group CN Aqueous Solubility < 0.1 g/L OR Group CN log Kow > 4.5 OR Group CN Molecular Weight > 290 g/mol OR Group CNHal Aqueous Solubility < 0.004 g/L OR Group CNHal Aqueous Solubility < 0.1 g/L OR Group CNHal log Kow > 3.8 OR Group CNHal Molecular Weight > 370 g/mol OR Group CNS Aqueous Solubility < 0.006 g/l OR Group CNS log Kow > 1.5 OR Group CNS log Kow > 3.6 OR Group CNS Melting Point > 200 C OR Group CNS Melting Point > 50 C by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "p"

Referential boundary:The target chemical should be classified as Not known precedent reproductive and developmental toxic potential by DART scheme v.1.0

Domain logical expression index: "q"

Referential boundary:The target chemical should be classified as Known precedent reproductive and developmental toxic potential OR Non-steroid nucleus derived estrogen receptor (ER) and androgen receptor (AR) OR Non-steroid nucleus derived estrogen receptor (ER) and androgen receptor (AR) >> 4-alkylphenol-like derivatives (2b-3) OR Piperazine-, dioxane-, morpholine-, tetrahydrothiopyran-like derivatives and cyclohexanamine (17c) OR Polyhalogenated benzene derivatives (8c) OR p-tert-Butyl-alpha-methylhydrocinnamic aldehyde (BMHCA)-like chemicals (9a) by DART scheme v.1.0

Domain logical expression index: "r"

Referential boundary:The target chemical should be classified as Bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "s"

Similarity boundary:Target: CCC(=O)OCC=Cc1ccccc1
Threshold=20%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "t"

Referential boundary:The target chemical should be classified as No alert found by Respiratory sensitisation

Domain logical expression index: "u"

Referential boundary:The target chemical should be classified as Acylation OR Acylation >> Direct acylation at a carbonyl with a leaving group OR Acylation >> Direct acylation at a carbonyl with a leaving group >> Phenyl acetates OR Acylation >> Ring opening acylation at a carbonyl OR Acylation >> Ring opening acylation at a carbonyl >> Anhydrides by Respiratory sensitisation

Domain logical expression index: "v"

Referential boundary:The target chemical should be classified as No alert found by in vitro mutagenicity (Ames test) alerts by ISS

Domain logical expression index: "w"

Referential boundary:The target chemical should be classified as Acyl halides OR Simple aldehyde by in vitro mutagenicity (Ames test) alerts by ISS

Domain logical expression index: "x"

Referential boundary:The target chemical should be classified as No superfragment by Superfragments ONLY

Domain logical expression index: "y"

Referential boundary:The target chemical should be classified as Not possible to classify according to these rules (GSH) by Protein binding potency

Domain logical expression index: "z"

Referential boundary:The target chemical should be classified as Moderately reactive (GSH) OR Moderately reactive (GSH) >> Alkyl 2-alkenoates (MA) OR Moderately reactive (GSH) >> Substituted 2-Alken-1-als (MA) by Protein binding potency

Domain logical expression index: "aa"

Referential boundary:The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3

Domain logical expression index: "ab"

Referential boundary:The target chemical should be classified as AN2 OR AN2 >> Michael-type addition on alpha, beta-unsaturated carbonyl compounds OR AN2 >> Michael-type addition on alpha, beta-unsaturated carbonyl compounds >> Four- and Five-Membered Lactones OR AN2 >> Shiff base formation after aldehyde release OR AN2 >> Shiff base formation after aldehyde release >> Specific Acetate Esters OR SN1 OR SN1 >> Nucleophilic attack after carbenium ion formation OR SN1 >> Nucleophilic attack after carbenium ion formation >> Specific Acetate Esters OR SN2 OR SN2 >> Acylation OR SN2 >> Acylation >> Specific Acetate Esters OR SN2 >> Alkylation, ring opening SN2 reaction OR SN2 >> Alkylation, ring opening SN2 reaction >> Four- and Five-Membered Lactones OR SN2 >> Nucleophilic substitution at sp3 Carbon atom OR SN2 >> Nucleophilic substitution at sp3 Carbon atom >> Specific Acetate Esters by DNA binding by OASIS v.1.3

Domain logical expression index: "ac"

Referential boundary:The target chemical should be classified as Esters (Acute toxicity) by US-EPA New Chemical Categories

Domain logical expression index: "ad"

Referential boundary:The target chemical should be classified as Neutral Organics OR Not categorized by US-EPA New Chemical Categories

Domain logical expression index: "ae"

Parametric boundary:The target chemical should have a value of log Kow which is >= 2.79

Domain logical expression index: "af"

Parametric boundary:The target chemical should have a value of log Kow which is <= 4.47

Interpretation of results:

other: not irritating

Conclusions:

Cinnamyl propionate was estimated to be not irritating to the eyes of New Zealand White rabbits.

Executive summary:

The ocular irritation potential of Cinnamyl propionate was estimated using OECD QSAR toolbox v3.3 with logPow as the primary descriptor.

Cinnamyl propionate was estimated to be not irritating to the eyes of New Zealand White rabbits.

 Based on the estimated results, Cinnamyl propionate can be considered to be not irritating to eyes and can be classified under the category “Not Classified” as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin Irritation:

In different studies,Cinnamyl propionate has been investigated for potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with human data for target chemical and its structurally similar read across substance,Cinnamyl acetate[CAS: 103-54-8]. The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

Various studies for Cinnamyl propionate were summarized in Food and Chemical Toxicology, 45, (2007), S82–S85; to assess the dermal irritation potential in humans and rabbits.

4% cinnamyl propionate in petrolatum was applied on the backs of 5 healthy, male volunteers in a 48-h closed patch test. Human volunteers were observed for signs of irritation. No irritation was observed after 48-hours in human volunteers. Hence, Cinnamyl propionate can be considered to be not irritating to human skin. Also, a test was performed as a part of acute dermal LD50 study. Ten rabbits received a single dermal application of neat cinnamyl propionate which was applied for 24 hours under occlusion. The rabbits were observed for signs of irritation till 21 days.  Moderate (4/10 rabbits) to slight erythema (5/10 rabbits) and slight edema (1/10 rabbits) were observed. Hence, Cinnamyl butyrate was considered to be mildly irritating to rabbit skin.

Even though rabbit study indicates that Cinnamyl propionate is mildly irritating to rabbit skin but human data concludes that it is not irritating to human skin. Since, in the rabbit study skin was exposed to higher concentration of Cinnamyl propionate than the normal use; there exists a possibility of Cinnamyl propionate being of not irritating to rabbit skin at lower concentrations. So, Cinnamyl propionate can be considered to be not irritating to skin.

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the dermal irritation potential was estimated forCinnamyl propionate. Cinnamyl propionate was estimated to be not irritating to the skin of New Zealand White rabbits.

Skin irritation effects were also estimated by four different models i.e, Battery, Leadscope, SciQSAR and CASE Ultra used within Danish QSAR database for Cinnamyl propionate. Based on estimation, No severe skin irritation effects were known when Cinnamyl propionate was exposed to rabbit skin.

The experimental and estimated data are in agreement with each other; substantiate the claim that Cinnamyl propionate is indeed not irritating to skin.

These above results are ably supported by the various studies summarized in Food and Cosmetics Toxicology, Volume 11, Issue 6, December 1973, Page 1063; for thestructurally similar read across substance, Cinnamyl acetate[CAS: 103-54-8].

Undiluted Cinnamyl acetate was applied under occlusion to the intact and abraded skin in rabbits and observed for signs of irritation (duration not mentioned).Cinnamyl acetate was not irritating to rabbit skin. Also,when tested on 25 human subjects at 5% in petrolatum in a 48-hr closed-patch test,cinnamyl acetateproduced no irritation.

 

Based on the available data for the target as well as read across substances and applying the weight of evidence approach,Cinnamyl propionate was not irritating to skin.Comparing the above annotations with the criteria of CLP regulation, test chemical can be classified under the category “Not Classified”.

 

Eye Irritation:

In different studies,Cinnamyl propionatehas been investigated for potential for ocular irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with predicted data for target chemical and its functionally similar read across substances,Methyl cinnamate[CAS: 103-56-4] and Methyl dihydrojasmonate[CAS: 24851-98-7]. The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the ocular irritation potential was estimated forCinnamyl propionate. Cinnamyl propionate was estimated to be not irritating to the eyes of New Zealand White rabbits.

This result is supported by the experimental study summarized in Food and Chemical Toxicology, 45 (2007), S113–S119; for thefunctionally similar read across substance,Methyl cinnamate[CAS: 103-56-4].0.1ml methyl cinnamate was instilled in one eye of 6 New Zealand White rabbits and observed for signs of irritation. The untreated eyes served as controls. Observations were made at 1, 4, 24, 48, 72 and 96 h and daily thereafter for a total of 7 days. Conjunctival irritation was observed in 1/6 rabbits for 24-h.Under the conditions of this study, methyl cinnamate was considered to be not irritating.

These results are also supported by the experimental study summarized in Food and Chemical Toxicology, 50 (2012), S517–S556; for thefunctionally similar read across substance, Methyl dihydrojasmonate[CAS: 24851-98-7]. The test was performed according to OECD 405 Guidelines.

Undiluted 0.1 ml methyl dihydrojasmonate was instilled into the conjunctival sac of 3 rabbits and observed for signs of irritation. The reactions were observed and scored after 1, 24, 48 and 72 hours of application. Scores were calculated for every animal as the mean following grading at 24, 48 and 72 h after instillation of the test material. No effects were observed on iris or cornea, slight conjunctival irritation was noted in all rabbits. The mean scores for redness in 2/3 rabbits was 0.3 and the effects got cleared by 48 hours. Hence, methyl dihydrojasmonate was considered not irritating to rabbit eyes.

Based on the available data for the target as well as read across substances and applying the weight of evidence approach,Cinnamyl propionate was not irritating to eyes.Comparing the above annotations with the criteria of CLP regulation, test chemical can be classified under the category “Not Classified”. 

Justification for classification or non-classification

Available studies for Cinnamyl propionate indicate that it is not likely to any cause irritation to skin and eyes.Hence, Cinnamyl propionate can be classified under the category “Not Classified” for skin and eyes as per CLP regulation.