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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2007-09-06 to 2007-10-05
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guidelineopen allclose all
according to guideline
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
equivalent or similar to guideline
EPA OPPTS 835.3110 (Ready Biodegradability)
equivalent or similar to guideline
EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
equivalent or similar to guideline
ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Details on test material:
- Name of test material (as cited in study report): 3',4',5'-Trifluoro-biphenyl-2-ylamine
- Physical state: Solid / dusky pink up to red
- Analytical purity: 99.67 area-%
- Lot/batch No.: 33569-66 eing.2
- Expiration date of the lot/batch: Unlimited
- Storage condition of test material: Storage at room temperature

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum: Collected on on 05 September 2007 from the wastewater treatment plant of Mannheim/Baden Württemberg (Germany).
- Preparation of inoculum for exposure: A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size of about 1
mm. After settling the supernatant was discarded and the sludge suspension was filled up with tap water. The sludge of the suspension was adjusted to a concentration of 6.0 g/L dry weight and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance. Finally the activated sludge suspension in the test vessels was pre-aerated for about 24 hours at a temperature of 22 ± 2 °C.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
16 mg/L
Based on:
test mat.
Initial conc.:
10 mg/L
Based on:
other: TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
- Composition of medium: According to guideline
- Test temperature: 22 ± 2 °C
- pH adjusted: yes, to 7.4 ± 0.2
- Aeration of dilution water: Aerated by bubble aeration with carbon dioxide free air.

- Culturing apparatus: 2 L incubation bottles
- Number of culture flasks/concentration: 2
- Details of trap for CO2: The bottles were connected to two serial scrubbing bottles (total volume 250 mL) filled with 100 mL 0.05 M sodium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes.

- Sampling procedure: The Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined and used for the
calculation of the produced carbon dioxide. After each sampling the second trap was moved forward and the new trap with fresh sodium hydroxide solution was placed into the second position. Each trap was analyzed separately.
- Sampling frequency: Usually twice a week

- 2 blank control assays
- 3 test substance assays
- 1 inhibition control test assay
- 1 reference substance assay

Reference substance
Reference substance:

Results and discussion

% Degradation
% degradation (CO2 evolution)
>= 10 - <= 20
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation degree of the reference substance after 14 days (% CO2/ThCO2): 78

Any other information on results incl. tables

Degradation degree in the inhibition control after 14 days (% CO2/ThCO2): 42

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
other: Poorly biodegradable