3',4',5'-trifluorobiphenyl-2-amine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3’,4’,5’-Trifluoro-biphenyl-2-ylamine
- Physical state: Solid, beige
- Analytical purity: 99.8%

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

-Test species and strain:Pseudokirchneriella subcapitata HINDAK, SAG 61.81
- Alge (on study day 0): A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 3 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
- Supplier: Collection of algal cultures in University of Göttingen/Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Nominal and measured concentrations:

The measured concentrations of the test item were between 88% and 107% of the nominal value at the start of exposure (0 hours) and 51% and 69% of the nominal value at the end of the exposure (72 hours). Therefore, all effect values given are based on the geometric mean measured test item concentration.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):
- Material, size, headspace, fill volume: Erlenmeyer flasks (nominal volume 250 mL) plugged with cotton wool plugs, 100 ml fill volume
- Initial cells density: 0.5 x 104 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8
- Light intensity and quality: Average 4900 lux (within ±15% variability) at a wave length of 400 - 700 nm

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

3.38 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.097 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes