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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Physical state: solid, red
- Analytical purity: > 99 %
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): The test substance is insoluble in water. Therefore the weighed portion was mixed with quartz sand and then formulated in artificial soil. Portions of about 750 g moistened substrate were given to the test pots.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Source: Jiang Su province Jurong Stock Farm
- Weight at test initiation (mean and range, SD): 300 - 600 mg

ACCLIMATION
- Acclimation period: 1 day before begin of exposure
- Acclimation conditions (same as test or not): same

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d

Test conditions

Test temperature:
19.8 - 20.3 °C
Moisture:
40 - 60 % WHC
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 1 L glass vessels covered with a lid
- Amount of soil or substrate: 750 g wt
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


OTHER TEST CONDITIONS
- Photoperiod: continous light
- Light intensity: 660 LUX


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality after 7 days and at the end of exposure

Nominal and measured concentrations:
Nominal test concentrations (mg/kg dry mass): 1000
Reference substance (positive control):
yes
Remarks:
2-chloroacetamide

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
LC50= 17.6 mg/kg dry matter

Applicant's summary and conclusion