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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from authoritative database

Data source

Reference
Reference Type:
other: Authoritative database
Title:
HSDB for the CAS: 108-99-6
Author:
U.S National Library of Medicine
Year:
2015
Bibliographic source:
Hazardous Substances Data Bank (HSDB), U.S National Library of Medicine, last updated 2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To assess the ocular irritation potential of 3-methylpyridine in rabbits
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylpyridine
EC Number:
203-636-9
EC Name:
3-methylpyridine
Cas Number:
108-99-6
Molecular formula:
C6H7N
IUPAC Name:
3-methylpyridine
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): 3-methylpyridine
- Molecular formula: C6H7N
- Molecular weight: 93.1283 g/mol
- Substance type: organic
- Physical state: liquid
- Smiles: c1(cccnc1)C
- InChI: 1S/C6H7N/c1-6-3-2-4-7-5-6/h2-5H,1H3
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-methylpyridine
- Molecular formula: C6H7N
- Molecular weight: 93.1283 g/mol
- Substance type: organic
- Physical state: liquid
- Smiles: c1(cccnc1)C
- InChI: 1S/C6H7N/c1-6-3-2-4-7-5-6/h2-5H,1H3

Test animals / tissue source

Species:
rabbit
Strain:
other: New Zealand albino
Details on test animals or tissues and environmental conditions:
sex: male

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
no data available
Duration of treatment / exposure:
single exposure
Observation period (in vivo):
7 or 14 days
Duration of post- treatment incubation (in vitro):
no data available
Number of animals or in vitro replicates:
no data available
Details on study design:
SCORING SYSTEM: Eye irritation was characterized by ocular irritation indices (OII).

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
14 d
Reversibility:
not specified
Remarks on result:
probability of severe irritation
Irritant / corrosive response data:
Eye irritation assessments showed that irritation was maximal either 1 hr or 1 day after exposure
Other effects:
Histological examination of the eyes showed keratitis lesions in corneal epithelial tissue, fibrillary edematous lamallae dissociation, and cellular inflammatory infiltration.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Conclusions:
Eye irritation assessments showed that irritation was maximal either 1 hr or 1 day after exposure. Histological examination of the eyes showed keratitis lesions in corneal epithelial tissue, fibrillary edematous lamallae dissociation, and cellular inflammatory infiltration. Over longer periods of time, which included recovery, 3-methylpyridine was considered to be severely irritating and causes irreversible damage to rabbit eyes.
Executive summary:

The ocular irritation potential of 3-methylpyridine was assessed in rabbits. Undiluted 3-methylpyridine was applied to the eyes of male New Zealand albino rabbits and observed for signs of irritation till 7 or 14 days.Following 7 or 14 days rabbits were killed and histological examinations were performed on the eyes. Eye irritation was characterized by ocular irritation indices (OII).

Eye irritation assessments showed that irritation was maximal either 1 hr or 1 day after exposure. Histological examination of the eyes showed keratitis lesions in corneal epithelial tissue, fibrillary edematous lamallae dissociation, and cellular inflammatory infiltration. Over longer periods of time, which included recovery, 3-methylpyridine was considered to be severely irritating and causes irreversible damage to rabbit eyes.