Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16.08.2017 - 20.08.2017
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Short term toxicity to aquatic invertebrates was performed according to the OECD guideline 203 in a static system.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: 2-amino-3,5-dimethylbenzene-1-sulfonic acid
- Molecular formula: C8H11NO3S
- Molecular weight: 201.245 g/mol
- Smiles notation: O=S(=O)(O)c1c(N)c(cc(C)c1)C
- InChl: 1S/C8H11NO3S/c1-5-3-6(2)8(9)7(4-5)13(10,11)12/h3-4H,9H2,1-2H3,(H,10,11,12)
- Substance type: Organic
- Physical state: Solid white powder
- Batch no: GI01LROB
- Storage condition: Ambient Temp (23 to 27 °C) √ (Store in a cool place. Keep containers tightly closed in a dry and well ventilated place)
- Solubility in water: 3049.2 mg/L(In house)
Analytical monitoring:
not specified
Details on sampling:
No data available
Vehicle:
not specified
Details on test solutions:
Method: The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively. Potable water (passed through reverse osmosis system) was used.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source:Koyal aquarium Nagpur, MS (India)
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD):average 1.8 cm
- Weight at study initiation (mean and range, SD):0.662 g (approx.)
- Method of breeding: No data available
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food during acclimation: No data available
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed): No mortality was observed after the exposure of 48 hrs.

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

FEEDING DURING TEST
- Food type: No data available
- Amount: No data available
- Frequency: Daily once
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Remarks on exposure duration:
No data availabe
Post exposure observation period:
24, 48, 72 and 96 hours
Hardness:
150.5
Test temperature:
23 °C
pH:
7.4
Dissolved oxygen:
7.7 (mg/l)
Salinity:
No data availabe
Conductivity:
No data availabe
Nominal and measured concentrations:
6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: plastic aquaria
- Type (delete if not applicable): No data
- Material, size, headspace, fill volume: plastic aquaria having the water capacity of 5liters filled with 2 liter of water containing test chemical and headspace of 3 liters.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- Type of flow-through (e.g. peristaltic or proportional diluter): No data
- Renewal rate of test solution (frequency/flow rate): 1 day before and during exposure
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: No data

TEST MEDIUM / WATER PARAMETERS: No data available
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16 hours light and 8 hours dark
- Light intensity: No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH, Dissolved oxygen, Temperature, Mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: No data available
- Test concentrations: 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
- Results used to determine the conditions for the definitive study: Mortality rate
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Nontoxic, no mortality was observed
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Non toxic
Details on results:
No abnormalities was observed
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
No data
Sublethal observations / clinical signs:

Fig:Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Fig: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

 6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

50

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 2-amino-3,5-dimethylbenzene-1-sulfonic acid on Zebra Fish was > 100 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were

6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours.

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations.

The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations. The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations.

The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.