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EC number: 201-811-4 | CAS number: 88-22-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16.08.2017 - 20.08.2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Short term toxicity to aquatic invertebrates was performed according to the OECD guideline 203 in a static system.
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material: 2-amino-3,5-dimethylbenzene-1-sulfonic acid
- Molecular formula: C8H11NO3S
- Molecular weight: 201.245 g/mol
- Smiles notation: O=S(=O)(O)c1c(N)c(cc(C)c1)C
- InChl: 1S/C8H11NO3S/c1-5-3-6(2)8(9)7(4-5)13(10,11)12/h3-4H,9H2,1-2H3,(H,10,11,12)
- Substance type: Organic
- Physical state: Solid white powder
- Batch no: GI01LROB
- Storage condition: Ambient Temp (23 to 27 °C) √ (Store in a cool place. Keep containers tightly closed in a dry and well ventilated place)
- Solubility in water: 3049.2 mg/L(In house) - Analytical monitoring:
- not specified
- Details on sampling:
- No data available
- Vehicle:
- not specified
- Details on test solutions:
- Method: The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations of 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L,respectively. Potable water (passed through reverse osmosis system) was used.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source:Koyal aquarium Nagpur, MS (India)
- Age at study initiation (mean and range, SD):3-4 months
- Length at study initiation (length definition, mean, range and SD):average 1.8 cm
- Weight at study initiation (mean and range, SD):0.662 g (approx.)
- Method of breeding: No data available
- Maintenance of the brood fish:
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food during acclimation: No data available
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed): No mortality was observed after the exposure of 48 hrs.
QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available
FEEDING DURING TEST
- Food type: No data available
- Amount: No data available
- Frequency: Daily once - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- No data availabe
- Post exposure observation period:
- 24, 48, 72 and 96 hours
- Hardness:
- 150.5
- Test temperature:
- 23 °C
- pH:
- 7.4
- Dissolved oxygen:
- 7.7 (mg/l)
- Salinity:
- No data availabe
- Conductivity:
- No data availabe
- Nominal and measured concentrations:
- 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: plastic aquaria
- Type (delete if not applicable): No data
- Material, size, headspace, fill volume: plastic aquaria having the water capacity of 5liters filled with 2 liter of water containing test chemical and headspace of 3 liters.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- Type of flow-through (e.g. peristaltic or proportional diluter): No data
- Renewal rate of test solution (frequency/flow rate): 1 day before and during exposure
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: No data
TEST MEDIUM / WATER PARAMETERS: No data available
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 16 hours light and 8 hours dark
- Light intensity: No data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH, Dissolved oxygen, Temperature, Mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: No data available
- Test concentrations: 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
- Results used to determine the conditions for the definitive study: Mortality rate - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Nontoxic, no mortality was observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Non toxic
- Details on results:
- No abnormalities was observed
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
Fig:Mortality and symptoms
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3hr
6hr
24hr
48hr
72hr
96hr
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Fig: Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3hr
6hr
24hr
48hr
72hr
96hr
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 2-amino-3,5-dimethylbenzene-1-sulfonic acid on Zebra Fish was > 100 mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were
6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours.
The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations.
The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.
Reference
Description of key information
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations. The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 2-amino-3,5-dimethylbenzene-1-sulfonic acid. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/l and Zebra fish were exposed to these concentration for 96 hours. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring for achieving test concentrations.
The result were recorded after 24, 48, 72 and 96 hours of the start of the experiment . . The lethal concentrations LC50 was = >100 mg/Lmg/L.The lethal concentrations LC0 was 100 mg/l, LC50 was > 100 mg/L and the LC100 was > 100 mg/l. Based on the LC50, it was concluded that the chemical was not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.
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