2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]vinyl]-1,3,3-trimethyl-3H-indolium hydrogen sulphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

07 Dec 1987 to 11 Dec 1987

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Justification for type of information:

Test material information missing: no details on composition of the test material available

Qualifier:

according to guideline

Guideline:

other: DIN38412 ("Testverfahren mit wasserorganismen (Gruppe L). Allgemeine hinweise zur planung, durchfuehrung und auswertung biologischer test-verfahren (L1)" und "Bestiumming der wirkung von wasserinhaltsstoffen auf fische - Fischtest (L15)")

Version / remarks:

June, 1982

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Basacryl Brilliantrot x 4G
- Batch No.: P. 1/86
- Degree of purity: Techn.
- Solubility in water: 236 g/L

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden Orfe
- Strain: golden variety
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt, FRG
- Body length: 6.2 cm (range: 5.7 - 6.9)
- Body weight: 2.7 g (range: 2.2 - 3.4)
- Corpulence factor of the batch: 1.2

HOUSING
- The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Photoperiod: 16 hours light and 8 hours dark
- Total hardness: about 2.5 mmol/L
- Acid capacity: about 5.5 mmol/L
- Oxygen content: >60% of maximum saturation
- pH: about 8.0
- Duration of housing and adaptation: about 6 weeks (adaptation period 3 days)
- Water temperature: 20 ± 1 °C
- Mortality during the last 2 weeks of housing: about 0.1%
- Mortality during the adaptation period: 0%
- Medical treatment: twice with 0.05 mg/L malachite green chloride; once with 10 mg/L tetracycline hydrochloride
- Diet: ad libitum growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, D-4770 Soest, FRG
- Withdrawel of food before exposure: 1 day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/L

Test temperature:

20 - 21 °C

pH:

7.8 - 8.1

Dissolved oxygen:

6.5 - 8.4 mg/L

Nominal and measured concentrations:

- Nominal test item concentrations: 0 (control), 100, 215, 464 and 1000 mg/L (based on range-finding study)

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass aquarium (30 cm x 22 cm x 24 cm)
- Aeration: slight
- Volume of water: 10 liters
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Loading (g fish/ L test water): 2.7

TEST MEDIUM
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11, draft sept. 1981, preparation from fully demineralized tap water (max. 10 µMHO), resaltingis carried out by addition of 294.0 mg/L CaCl2·2H2O, 123.3 mg/L MgSO4·7H2O, 64.8 mg/L NaHCO3 and 5.8 mg/L KCl. Continuous aeration with oil-free air.

WATER PARAMETERS OF TEST WATER READY FOR USE
- Total hardness: 2.5 mmol/L
- Acid capacity: 0.8 mmol/L
- Ratio Ca/Mg ions: 4 : 1
- Ratio Na/K ions: 10 : 1
- pH: about 8.0

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

EFFECT PARAMETERS MEASURED:
- Survival and symptoms after 1, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Reason for selection of the concentrations: based on the results of a range finding study with test concentrations 0, 10, 100, 1000 and 10,000 mg/L (LC50 after 96 h: between 100 and 1000 mg/L)
- Spacing factor for test concentrations: 2.2

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

MORTALITY
No mortality was observed in the control and 100 mg/L test concentration. At the test concentration of 215 mg/L 2 fish (20%) were dead after 24 hours exposure and 3 fish (30%) were dead after 96 hours. At the test concentration of 464 mg/L all 10 fish were dead after 4 hours. At the highest tested concentration all fish had died within 1 hour exposure. See 'Any other information on results incl. tables'.

SYMPTOMS:
No symptoms of toxicity were observed in any of the test concentrations.

Results with reference substance (positive control):

- LC50 after 48h: about 31 mg/L (determined 30 Nov 1987), this lethal concentration corresponds to the normal sensitivity
- Results with reference substance were valid

Reported statistics and error estimates:

Determination or calculation of the median lethal concentration (LC 50) and, if possible the LC 5 and the LC 95 using the probit analysis.

Sublethal observations / clinical signs:

Table: Results on fish survival and symptoms

Nominal conc. (mg/L)	Number of fish	Dead fish after
		1h	4h	24h	48h	72h	96h
100	10	0	0	0	0	0	0
215	10	0	0	2	2	2	3
464	10	0	10	10	10	10	10
1000	10	10	10	10	10	10	10
0	10	0	0	0	0	0	0
Nominal conc. (mg/L)		Symptoms
		1h	4h	24h	48h	72h	96h
100	No symptoms detectable
215
464
1000
0

Explanation of symptoms:

a=apathy, b=abdominal distension, e=exophthalmos, f=escapereflex, h=hyperreflexia, k=convulsions, l=gasping, n=narcotic-likestate, t=tumbling, u=restlessness, v=discoloration, w=headstand, x=accelerated respiration

 

 

Table: (Additional) effect values

LC 50 (mg/L; nominal concentrations) after:
1h	greater	460 (mg/L)	(1% significance level)
	lower	1000 (mg/L)	(1% significance level)
4h	greater	220 (mg/L)	(1% significance level)
	lower	460 (mg/L)	(1% significance level)
24h	greater	220 (mg/L)	(10% significance level)
	lower	460 (mg/L)	(1% significance level)
48h	greater	220 (mg/L)	(10% significance level)
	lower	460 (mg/L)	(1% significance level)
72h	greater	220 (mg/L)	(10% significance level)
	lower	460 (mg/L)	(1% significance level)

                       

- No Observable Effect Concentration: 100mg/L

- Maximum concentration causing no mortality: 100 mg/L

- Minimum concentration causing 100% mortality: 464 mg/L

 

Validity criteria fulfilled:

yes

Description of key information

The 96-h LC50 value for Basacryl-brilliantrot x 4G is ca. 250 mg/L in freshwater fish (Leuciscus Idus L.).

Additional information

The short-term toxicity of Basacryl-brilliantrot x 4G to freshwater fish was determined in a study according to DIN 38 412. In this study, groups of 10 Golden Orfe (L. idus) were exposed to nominal test substance concentrations of 0 (control), 100, 215, 464 and 1000 mg/L for 96 hours under static conditions. Test concentrations were not analytically verified. Incidences of mortality and clinical effects were recorded after 1, 4, 24, 48, 72 and 96 hours exposure. No clinical effects were observed. No mortality was observed in the control and 100 mg/L test concentration. At the test concentration of 215 mg/L 2 fish (20%) were dead after 24 hours exposure and 3 fish (30%) were dead after 96 hours. At the test concentration of 464 mg/L all 10 fish (100%) were dead after 4 hours. At the highest tested concentration all fish had died within 1 hour exposure. Based on these findings the 96-h LC50 is determined at ca. 250 mg/L.