Violanthrene-5,10-dione

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1984

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

no guideline available

Principles of method if other than guideline:

- Principle of test: Biochemical oxygen demand determined after 5 d at 20°C inoculation
- Parameters analysed / observed: oxygen content at 0 h and after 5 d inoculation

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Duration of test (contact time):

5 d

Parameter:

% degradation (O2 consumption)

Remarks on result:

other: BOD5

Parameter:

BOD5

Value:

31 mg O2/g test mat.

Parameter:

COD

Value:

1 935 mg O2/g test mat.

Key result

Parameter:

BOD5*100/COD

Value:

2 mg O2/g test mat.

Validity criteria fulfilled:

not applicable

Interpretation of results:

not readily biodegradable

Conclusions:

The BSB5/COD ratio is 0.02. Therefore the substance is not considered to be readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Version / remarks:

1981

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): ARA Birsfelden

Duration of test (contact time):

28 d

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

ThOD (test substance): 2.6 mg O2 / mg substance

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

St. dev.:

0

Sampling time:

26 d

Details on results:

The toxicity control did not indicate any inhibitory effect on activated sluge. The degradation rate was determined at 109% after 26 d based on O2 consumption.

Parameter:

BOD5

Value:

< 1 mg O2/g test mat.

Parameter:

COD

Value:

859 mg O2/g test mat.

Results with reference substance:

82% degradation after 26 d based on O2 consumption

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

There was no biodegradation observed in an OECD 302C inherent biodegradabality test (0% after 26 d based on O2 consumption). The test substance is not considered to be inherently biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1979

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information given on negative control and validity criteria.

Qualifier:

according to guideline

Guideline:

other: Modified OECD Confirmatory Tests

Version / remarks:

The test was conducted according to SOP A-12 (1974-09-01).

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Duration of test (contact time):

39 d

Initial conc.:

20 mg/L

Based on:

TOC

Initial conc.:

59.7 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: TOC content of medium: 118 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 1


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Key result

Parameter:

% degradation (TOC removal)

Value:

91

St. dev.:

6

Sampling time:

21 d

Parameter:

BOD5

Value:

150 mg O2/g test mat.

Remarks on result:

other: Test item conc. 20 mg/L

Parameter:

COD

Value:

1 008 mg O2/g test mat.

Remarks on result:

other: Test item conc. 10 mg/L

Validity criteria fulfilled:

not specified

Interpretation of results:

inherently biodegradable

Conclusions:

The substance was found to be inherently biodegradable (91% after 21 d based on TOC removal).

Description of key information

Based on a weight-of-evidence approach with several BOD/COD measurements, a Confirmatory OECD 2 test (inherent biodegradability) and an OECD 302C MITI II (inherent biodegradability) which is the most reliable source, the substance is considered to be neither inherently nor readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed