Reaction products of acetic acid with formaldehyde and a mixture of 1-, 2-, 3-,octene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

EC Number:

242-640-5

EC Name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

Cas Number:

18871-14-2

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2,4-dideoxy-2-pentylpentitol

Constituent 2

Reference substance name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

EC Number:

253-863-2

EC Name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

Cas Number:

38285-49-3

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2-butyl-2,4-dideoxy-4-methylpentitol

impurity 1

Reference substance name:

3-pentyltetrahydro-2H-pyran-4-ol

Cas Number:

24646-98-8

Molecular formula:

C10H20O2=

IUPAC Name:

3-pentyltetrahydro-2H-pyran-4-ol

impurity 2

Reference substance name:

Likely acetylated constituents, MW 214

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents, MW 214

impurity 3

Reference substance name:

Likely acetylated constituents

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents

impurity 4

Reference substance name:

Unknown constituent, MW 172

Molecular formula:

n/a

IUPAC Name:

Unknown constituent, MW 172

Test material form:

liquid

Details on test material:

UVCB substance

Specific details on test material used for the study:

Batch number: PE00138468

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage was used. The sludge is collected in the morning, washed three times in the mineral medium and kept aerobig until being used on the same day.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

70

Sampling time:

28 d

Details on results:

The 10 day window criterion was not fulfilled (16% biodegradation on day 4 and 47% on day 14).

Any other information on results incl. tables

Jasmonyl LG did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Executive summary:

Jasmonyl LG undergoes 70% biodegradation after 28 days in the test conditions. The 10 -day window criterion is not fulfilled (16% biodegradation on day 4 and 48% on day 14). However, Jasmonyl LG is a complex mixture composed of strucutrally related constituents that are expected to have similar biodegradation potential. The degradation curve will therefore be the sum of many growth curves and the 10 -day window should be ignored as a pass / fail criterion for Jasmonyl LG.

Therefore, Jasmonyl LG and underlying constituents should be considered to be readily biodegradable according to this test based on the biodegradation percentage of 70% at day 28.