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EC number: 210-283-4 | CAS number: 611-92-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12.12.2016-10.02.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- The temperature was not in the prescribed range (20 ± 2 °C) during whole testing. The prescribed range was exceeded twice by about 0.4 and 0.1 °C in the preliminary test. This increase of the temperature takes only short period of time (2 hours).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,3-dimethyl-1,3-diphenylurea
- EC Number:
- 210-283-4
- EC Name:
- 1,3-dimethyl-1,3-diphenylurea
- Cas Number:
- 611-92-7
- Molecular formula:
- C15H16N2O
- IUPAC Name:
- 1,3-dimethyl-1,3-diphenylurea
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Preparation of Sample Solutions:
The test substance is poorly soluble in dilution water at concentrations needed for the test. For this reason the preliminary experiment was performed for finding suitable period for achieving the highest solubility.
For the preliminary experiment the solutions were prepared as follows: 100 mg of the test substance in 1000 mL of dilution water was stirred for period 3, 24, 48 and 72 hours on a shaft stirrer, subsequently filtered through 0.45 μm filter and the concentrations of the test substance in resulted solutions were determined.
Test solutions
- Details on test solutions:
- The stock solutions of the test substance were prepared in the dilution water. Due to lower solubility of the test substance were prepared two stock solutions for the preliminary and definitive test. 50 and 100 mg of the test substance was weighed into 1 000 mL of the dilution water for the preliminary test and 80 and 100 mg of the test substance was weighed into
1 000 mL of the dilution water for the definitive test No.2. The stock solutions were stirred for period 72 hours and subsequently filtered through 0.45 μm filter.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: own laboratory breeding.
- Age at study initiation: young Daphnia born in 24h
- Method of breeding: aperiodic parthenogenesis
- Feeding during test: no
ACCLIMATION
- Acclimation period: 48h
- Acclimation conditions: same as test
- Type and amount of food: a mixture of algae
- Health during acclimation: mortality control, healthy
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Ca+Mg ions
2.54 mmol/L preliminary study
2.52 mmol/L definitive test - Test temperature:
- 20±2°C
- pH:
- 7.9
- Dissolved oxygen:
- 8.5 mg//L preliminary study
8.6 mg/L definitive test - Conductivity:
- 2.26 µS.cm-1 preliminary study
0.88 µS.cm-1 definitive test - Nominal and measured concentrations:
- 100, 50, 10, 5, 1 mg/L preliminary test
100, 71, 51, 36, 26, 19 and 14mg/L definitive test - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, 50 ml
- Type: open
- Aeration: without
Number of test animals: 20 for each concentration and control
Number of replicates (per concentration): 2
Number of test animals per replicate: 10
Volume of test substance solution: 50 mL per 10 individuals
Dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1000 mL of deionized water.
A. 117.6 g CaCl2.2H20 in 1 L of deionized water
B. 49.3 g MgSO4.7H2O in 1 L of deionized water
C. 25.9 g NaHCO3 in 1 L of deionized water
D. 2.3g KCl in 1 L of deionized water - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 77.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 64.7 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Reference test
The sensitivity of the test species and correctness of test performance is periodically verified
in six-month period by testing with the reference substance, potassium dichromate.
The results of the verification test with K2Cr2O7, carried out in period from 09.11. to 11.11.2016 are the following:
24 hour – EC50 = 1.34 mg·L-1 (95% confidence limit: 1.15 – 1.67 mg·L-1)
48 hour – EC50 = 0.74 mg·L-1 (95% confidence limit: 0.66 – 0.84 mg·L-1)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 per cent
of the nominal or measured initial concentration throughout the test, the results can be based
on nominal or measured initial values.
Because concentrations of the test substance have been satisfactorily maintained
within ± 20 percent of the measured initial concentration throughout the test, the nominal concentrations were used for all evaluations and results.
24 hour – EC50 = 77.3 mg·L-1 (nominal concentration)
(95% confidence limit: 63.4 – 105.2 mg·L-1)
48 hour – EC50 = 64.7 mg·L-1 (nominal concentration)
(95% confidence limit: 54.9 – 80.4 mg·L-1)
24 hour – EC0 = 19 mg·L-1 (nominal concentration)
48 hour – EC0 = 19 mg·L-1 (nominal concentration)
24 hour – EC100 ˃ 100 mg·L-1 (nominal concentration)
48 hour – EC100 ˃ 100 mg·L-1 (nominal concentration) - Executive summary:
The test substance, Methylcentralit, was tested in acute immobilisation test on Daphnia magna.
The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
Since the test substance was poorly soluble in dilution water, the stock solutions were stirred for period 72 hours and subsequently filtered through 0.45 μm filter.
The preliminary test was performed in a range of the test substance nominal concentrations
1 – 100 mg·L-1.Based on toxicityof the test substance found in the preliminary test, the definitive test was performed inappropriate concentration range.The analytical results showed, that the test substance Methylcentralit was sufficiently stable in dilution water in the conditions of the test, therefore the definitive test was performed as static one.
The definitive test was performed in a range of the test substance nominal concentrations
14 – 100 mg·L-1. Samples for analytical determination were taken at the beginning and at the end of the test.The definitive test was performed twice.In the first definitive test the result of analytical determinationof concentration of the test substance in the highest concentration (100 mg·L-1)was different from theanalytical determination of concentration of the test substancein the preliminary test and this concentration was not maintained within ± 20 percent of the nominal concentration.Therefore the definitive test was repeated (definitive test No.2).
The definitive test No.2 was used for all evaluation and results.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based
on nominal or measured initial values.The nominal concentrations were used for all evaluations and results.
Test results:
24 hour – EC50= 77.3 mg·L-1 (nominal concentration)
(95% confidence limit: 63.4 – 105.2 mg·L-1)
48 hour – EC50= 64.7 mg·L-1 (nominal concentration)
(95% confidence limit: 54.9 – 80.4 mg·L-1)
24 hour – EC0= 19mg·L-1 (nominal concentration)
48 hour – EC0=19mg·L-1 (nominal concentration)
24 hour – EC100˃ 100 mg·L-1 (nominal concentration)
48 hour – EC100˃ 100 mg·L-1 (nominal concentration)
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