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EC number: 267-184-4 | CAS number: 67801-64-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
One valid enhanced ready and two ready studies have been performed on the test substance.
Two ready studies (MITI 1 and Closed bottle test) failed to find significant biodegradation within 28 days therefore a further enhanced ready test was performed on the test substance using an OECD 301F (Hammesfahr, 2016) over a period of 60 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure with a test item loading rate of 103.5 mg/L corresponding to an oxygen demand of about 253.8 mg/L (ThODNH4). As a reference item sodium benzoate (103.3 mg/L corresponding to an oxygen demand of about 172.1 mg/L(ThODNH4)) was tested simultaneously under the same conditions as the test item. It functioned as a procedure control. Degradation rate of test item calculated by the oxygen consumption of the aerobic activated sludge microorganisms after 60 days of incubation.
All validity criteria were met. Degradation of the positive control was 76% after 14 and 28 days and to 78% after 60 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
The degradation rate of the test substance did not reach 60% within 28 days. Therefore, it is considered to be not readily biodegradable. The degradation rate did not reach 60% after 60 days although some inhibition of basal respiration rate was observed in the test solution flasks compared to the control suggesting potential toxicity and increase in lag time. From day 30, mineralisation started to occur and remained linear until the end of the study where 32% biodegradation was recorded without a plateau being reached.
While non-Persistency of the degradation products cannot be assumed based on this evidence, 32% degradation within 60 days is sufficient to assume 100% primary biodegradation of the test substance as well as at least partial degradation of some of the metabolites.
In a third study using an OECD 301D method no biodegradation was observed over 28 days however, despite the reference reaching the required threshold, very low basal respiration was observed over the study in the controls suggesting low microbial activity for an OECD 301D so the lack of biodegradation observed in this study should be considered only as supporting evidence that the test substance does not rapidly degrade.
The substance may be considered inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
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