[3R-(3α,3aβ,6α,7β,8aα)]-octahydro-3,6,8,8-tetramethyl-1H-3a,7-methanoazulen-5-yl acetate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[3R-(3α,3aβ,6α,7β,8aα)]-octahydro-3,6,8,8-tetramethyl-1H-3a,7-methanoazulen-5-yl acetate

EC Number:

201-036-1

EC Name:

[3R-(3α,3aβ,6α,7β,8aα)]-octahydro-3,6,8,8-tetramethyl-1H-3a,7-methanoazulen-5-yl acetate

Cas Number:

77-54-3

Molecular formula:

C17H28O2

IUPAC Name:

[3R-(3α,3aβ,6α,7β,8aα)]-octahydro-3,6,8,8-tetramethyl-1H-3a,7-methanoazulen-5-yl acetate

Test solutions

Vehicle:

yes

Details on test solutions:

The stock solution used in the range-finding test was prepared by dissolving 1000 mg of the test item in volume completed to 1000 mL with dilution water, resulting in a concentration of 1000 mg.L-1. To assist solubilization, ultrasonic dispersion was also applied for 20 minutes.

The stock solution used in the definitive test was prepared by dissolving 1000 mg of the test item in volume completed to 1000 mL with dilution water, resulting in a concentration of 1000 mg.L-1. To assist solubilization, ultrasonic dispersion was also applied for 20 minutes.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The fish were obtained from supplier Aquario Way (Taquaral - Campinas/SP), maintained and acclimated under laboratory-controlled conditions. The fish were healthy and free from any visible disease and abnormalities during the maintenance and acclimation periods

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Fish in the treatment groups and control group were observed for general symptoms at 24, 48, 72 and 96 hrs after exposure and the mortality was recorded.

Test conditions

Test temperature:

21 ~ 25 °C

pH:

7.6 ~ 7.8

Dissolved oxygen:

7.9 ~ 8.1 mg/L

Nominal and measured concentrations:

6.25; 12.5; 25; 50 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 15.61 mg/L

Details on results:

The 24, 48, 72, and 96 hours cumulative mortality responses of Danio rerio to each test item concentration are shown in tables 5 and 6 for the range-finding and definitive tests, respectively. No mortality or sublethal effects were observed in the control group for the range-finding and definitive tests, showing that validity of the test was achieved. The 24 hours mortality of Danio rerio in each test concentration in the reference test with potassium dichromate is presented in the Table 7.

The values of 24, 48, 72 and 96-hour LC50 of Cedryl Acetate were 28.72; 15.39; 15.61 and 15.61 mg.L-1, respectively. The 24-hour LC50 value for the reference item, potassium dichromate, was 253.30 mg.L-1, demonstrating that the sensitivity of the fish was within the accepted range (ISO, 1982). All values of LC50 of both the test and reference items and their respective confidence limits are shown in Table 8.

The highest concentration that caused no lethal effect on fish was not determined and the lowest concentration that killed 100% of fish was 25 mg.L-1, in 96 hours of exposure period (Figure 4).

No sublethal effects on fish were observed at any of the tested concentrations.

Applicant's summary and conclusion

Conclusions:

The 96-hour LC50 value of Cedryl Acetate was 15.61 mg.L-1. The highest concentration that did not cause lethal effect on fish was not determined1, and the lowest concentration that caused 100% mortality on the fish population was 25 mg.L-1, in 96 hours of exposure period.