Ammonium hexafluorosilicate

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo insect germ cell study: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Publication date - 11 May 1981.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)

GLP compliance:

not specified

Type of assay:

Drosophila SLRL assay

Test material

Test material information

Constituent 1

Reference substance name:

disodium;hexafluorosilicon(2-)

Cas Number:

16893-85-9

Molecular formula:

Na2SiF6

IUPAC Name:

disodium;hexafluorosilicon(2-)

Specific details on test material used for the study:

Test material was obtained from Fisher Scientific Co., Fair Lawn, NJ (U.S.A.);

Test animals

Species:

Drosophila melanogaster

Strain:

other: The Berlin K (wild-type) and Basc strains were used.

Administration / exposure

Route of administration:

oral: feed

Vehicle:

2 % Tween 80

Details on exposure:

In Drosophila one dose close to the LD50 (0.25 mM) was applied by the adult feeding method in 5 % saccharose.

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0.25 other: mM

Doses / concentrations 2

Dose / conc.:

42.02 other: mg

Remarks:

Dose was not specified in terms of 'per kg'

Results and discussion

Test results

Key result

Sex:

not specified

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

Of the tested chromosomes, only a small portion (brood 1: 3 out of 1226, brood 2: 1 out of 1212 and brood 3: 2 out of 1236) were confirmed as sex-linked recessive lethals.

Applicant's summary and conclusion

Conclusions:

Sodium hexafluorosilicate did not induce mutations/was not mutagenic in the Drosophila sex-linked recessive lethal assay.

Executive summary:

Sodium hexafluorosilicate was evaluated for the potential to induce mutations in a Drosophila melanogaster sex linked recessive lethal assay. Drosophila melanogaster strains: Berlin K (wild-type) and Basc, were used. In the tested Drosophila, one dose close to the LD50 (0.25 mM or 42.02 mg) was applied by the adult feeding method in 5 % saccharose. About 1200 X-chromosomes (brood 1: 1226, brood 2: 1212 and brood 3: 1236) were tested per experiment in each of 3 successive broods (3-3-4 days). In repeat experiments, sometimes only single broods were tested. F: progeny cultures with 2 or fewer wild-type males were routinely retested in the F3 generation to confirm X-linked recessive lethal mutations (RLs). Mosaics were not counted. "Clusters" of 2 were included because their occurrence was compatible with statistical expectation of independent origin. Of the tested chromosomes, only a small portion (brood 1: 3 out of 1226, brood 2: 1 out of 1212 and brood 3: 2 out of 1236) were confirmed as sex-linked recessive lethals. Hence based on the above findings, it can be concluded that sodium hexafluorosilicate did not induce mutations/was not mutagenic in the Drosophila sex-linked recessive lethal assay.