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EC number: 221-967-7 | CAS number: 3296-90-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and appropriate guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- US EPA, Method: HG-Gene MUta-S. typhimurium; the Salmonella typhimurium reverse mutation assay, 1984
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2-bis(bromomethyl)propane-1,3-diol
- EC Number:
- 221-967-7
- EC Name:
- 2,2-bis(bromomethyl)propane-1,3-diol
- Cas Number:
- 3296-90-0
- Molecular formula:
- C5H10Br2O2
- IUPAC Name:
- 2,2-bis(bromomethyl)propane-1,3-diol
- Details on test material:
- Substance: FR -522 Purified (2,2-bis(bromomethyl)-1,3-propanediol), Purity: 99.5%,
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- not relevant
- Additional strain / cell type characteristics:
- other: Strains TA 98 and TA 100 possess a plasmid (pKM101) which introduce an error prone repair process resulting increased sensitivity to mutagens
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver preparations from Aroclor 1254 induced rats and uninduced syrian hamsters.
- Test concentrations with justification for top dose:
- Preliminary toxicity test: Solvent, 5, 50, 500, 5000 microgram/plate in the presence and absence of liver S-9 mix/Hamster S-9 mix
Mutation tests: Solvent, 0, 50, 150, 500, 1500, 5000 microgram/plate in the presence and absence of liver S-9 mix/Hamster S-9 mix - Vehicle / solvent:
- Solvent: Dimethylsulphoxide
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethylsulphoxide (DMSO)
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- In the absence of S-9 mix
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- In the absence of S-9 mix
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- In the absence of S-9 mix
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- In the presence of S-9 mix
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- congo red
- Remarks:
- In the presence of S-9 mix
- Details on test system and experimental conditions:
- Test Type: Reverse Mutation Assay
System of testing: Bacterial
Species/Strain: Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537
Metabolic activation: S9-mix,Rat liver cells, Aroclor induced; S9-mix,Hamster liver cells, uninduced
Test Design
Number of replicates: 3
Positive Controls: N-ethyl -N’-nitroso-N-nitrosoguanidine (TA100, TA1535, -S9)
9-aminoacridine (TA1537, -S9)
2-nitrofluorene (TA98, -S9); 2-aminoanthracene (TA98, TA100, TA 1535, TA1537, +S9)
Congo red (TA98, +S9)
Negative Control: Solvent vehicle
Solvent: Dimethylsulfoxide
(see attached experimental procedure) - Evaluation criteria:
- See attached document (assessment of results)
- Statistics:
- see attached document (statistical results AMES 001)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Remarks:
- Not tested
- Metabolic activation:
- not applicable
- Genotoxicity:
- not specified
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- other: Not mutagenic when tested without S9. Mutagenic when tested with S9.
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- other: Not mutagenic when tested without S9. mutagenic when tested with S9
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Result: Positive
Cytotoxic concentration -With metabolic activation: > 5000 μg/plate -Without metabolic activation:> 5000 μg/plate
Genotoxic effects:
With metabolic activation: Large dose-related increases in revertant colony numbers were observed with strain TA1535.
These increases were observed only in the presence of hamster S-9 mix and were largest in the presence of the 30% mix.
A smaller increase was also observed with strain TA100 in the presence of 30% hamster S-9 mix.
Without metabolic activation:None - Remarks on result:
- other: other: Preliminary toxicity test
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
see attached table (statistical results)
Remark: S-9 is usually only prepared from a rat previously treated with a compound (Aroclor) known to induce a high level of enzymic activity. However in this study S-9 mix using liver fraction from uninduced Syrian hamsters was also used as it was suspected that the test substance may be metabolised to a mutagen by hamster S-9 but not by rat.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
It is concluded that, when tested in dimethylsulfoxide, the test substance shows no evidence of mutagenic activity in the absence or presence of rat S-9 mix. The test substance shows clear evidence of mutagenic activity with strains TA1535 and TA100 in the presence of hamster S-9 mix. - Executive summary:
It is concluded that, when tested in dimethylsulfoxide, the test substance shows no evidence of mutagenic activity in the absence or presence of rat S-9 mix. The test substance shows clear evidence of mutagenic activity with strains TA1535 and TA100 in the presence of hamster S-9 mix.
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