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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authoritatve database

Data source

Reference
Reference Type:
publication
Title:
The Metabolism of Potassium Dodecyl [35S]Sulphate in the Rat
Author:
W. H. B. DENNER, A. H. OLAVESEN, GILLIAN M. POWELL AND K. S. DODGSON
Year:
1969
Bibliographic source:
Biochem. J. (1969) 111, 43

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
Metabolism of Potassium dodecyl sulfate in rats
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium dodecyl sulphate
EC Number:
225-190-4
EC Name:
Potassium dodecyl sulphate
Cas Number:
4706-78-9
Molecular formula:
C12H25KO4S
IUPAC Name:
potassium dodecyl sulfate
Test material form:
solid: crystalline
Details on test material:
- Name of test material : potassium dodecyl sulfate
- Molecular formula : C12H25KO4S
- Molecular weight : 304.49 g/mole
- Smiles notation : S(OCCCCCCCCCCCC)(=O)([O-])=O.[K+]
- InChl : 1S/C12H26O4S.K/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid
Specific details on test material used for the study:
- Name of test material : Potassium dodecyl sulfate
- Molecular formula : C12H25KO4S
- Molecular weight : 304.49 g/mole
- Smiles notation : S(OCCCCCCCCCCCC)(=O)([O-])=O.[K+]
- InChl : 1S/C12H26O4S.K/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
- Substance type: Organic
- Physical state: Solid
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
other: M.R.C hooded
Sex:
male/female
Details on test animals or test system and environmental conditions:
Adult male and female M.R.C. hooded rats were used throughout unless otherwise stated. Intraperitoneal injections of the esters were carried out
while the animals were under ether anaesthesia. The animals were placed in metabolism cages that permitted separate collection of urine and faeces, and were
allowed water without restriction and food after 24hr. Urine samples were collected after 6, 12, 24 and 48hr., funnel washings being added to the appropriate sample.

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
Free-ranging male and female rats were injected intraperitoneally with potassium dodecyl [35S] sulphate (1 mg. dissolved in 1 ml. of water). Male rats received potassium dodecyl [35S]sulphate orally
Duration and frequency of treatment / exposure:
48 hr
Doses / concentrations
Remarks:
1 mg/ml
No. of animals per sex per dose / concentration:
6
Control animals:
no
Positive control reference chemical:
No data
Details on study design:
No data
Details on dosing and sampling:
No data
Statistics:
No data

Results and discussion

Preliminary studies:
No data
Main ADME resultsopen allclose all
Type:
absorption
Results:
potassium dodecyl [35S]sulphate administered intraperitoneally & orally was absorbed in the rats
Type:
distribution
Results:
Distribution of radioactivity was found in urine, faeces and carcasses of the rats.
Type:
metabolism
Results:
The chemical is initially metabolized to butyric acid 4[35S]-sulphate, which may then be desulphated with the liberation of inorganic [35S]sulphate. It is assumed that the same degradative pathway may operate in both micro-organisms and in man.
Type:
excretion
Results:
Bulk of the radioactivity was excreted in the urine within 12 hr, approximately 17% being eliminated as inorganic [35S]sulphate.

Toxicokinetic / pharmacokinetic studies

Details on absorption:
No data
Details on distribution in tissues:
No data
Details on excretion:
No data

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
butyric acid 4[35S]-sulphate & inorganic [35S]sulphate.

Bioaccessibility (or Bioavailability)

Bioaccessibility (or Bioavailability) testing results:
No data

Any other information on results incl. tables

Organs exhibiting radioactivity: Liver, kidney

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): low bioaccumulation potential based on study results
In a study to investigate the metabolism of Potassium Dodecyl [35S] Sulphate in the Rat, the chemical was absorbed after oral and intraperitoneal administration. It was also metabolized to butyric acid 4[35S]-sulphate, which may then be desulphated with the liberation of inorganic [35S] sulphate. In addition, bulk of the radioactivity was excreted in the urine within 12 hr, approximately 17% being eliminated as inorganic [35S] sulphate. Considering this rapid elimination of the chemical out of the living system, it can be concluded that the chemical is likely to have low bio-accumulation potential.
Executive summary:

In a study to investigate the metabolism of Potassium Dodecyl [35S] Sulphate in the Rat, the chemical was absorbed after oral and intraperitoneal administration. It was also metabolized to butyric acid 4[35S]-sulphate, which may then be desulphated with the liberation of inorganic [35S] sulphate. In addition, bulk of the radioactivity was excreted in the urine within 12 hr, approximately 17% being eliminated as inorganic [35S] sulphate. Considering this rapid elimination of the chemical out of the living system, it can be concluded that the chemical is likely to have low bio-accumulation potential.