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No short-term terrestrial test data is available for diquat C16 -18. One long-term terrestrial test has been performed with diquat C16 -18.

The effects of diquat C16 -18 on mortality, biomass and reproduction of Eisenia fetida was tested according to OECD 222 under a static exposure for 28 days. Natural soil (Lufa 2.2) was used as substrate. The concentrations applied were 62.5, 125, 250, 500, and 1,000 mg test substance/kg soil dry weight.

The test is valid without restrictions and the results are considered to be reliable. After 28 days of exposure, no effects on survival of the adult worms were observed in all concentrations. After the following four weeks, no significant effects on the reproduction rate (average number of juveniles) were observed compared to the control. Hence, the NOEC is 1000 mg/kg dw soil i.e. 945 mg A.I./kg dw.

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