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EC number: 228-544-6 | CAS number: 6291-84-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenic Activity from Nitrosation of Oligoamines
- Author:
- Michael Murphey-Corb, Hay-Long Kong, and Michael L. Murray
- Year:
- 1 983
- Bibliographic source:
- Environmental Mutagenesis 5101-109 (1983)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of N-Methyl-1,3-propanediamine in Salmonella typhimurium LT2 strains TA 1950 (hisG46, uvrB} ; TS24 (hisG46,recA); TA1537 (hisC3076, uvrB, $a); TA1538 (hisD3052, uvrB, $a}; TA1952(hisC3076, uvrB); and mutant hisG46.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-aminopropylmethylamine
- EC Number:
- 228-544-6
- EC Name:
- 3-aminopropylmethylamine
- Cas Number:
- 6291-84-5
- Molecular formula:
- C4H12N2
- IUPAC Name:
- (3-aminopropyl)(methyl)amine
- Test material form:
- liquid
- Details on test material:
- - Name of test material : (3-aminopropyl)(methyl)amine; 3-Amino-1-methylaminopropane
- Molecular formula : C4H12N2
- Molecular weight : 88.1528 g/mol
- Smiles notation : N(CCCN)C
- InChl : 1S/C4H12N2/c1-6-4-2-3-5/h6H,2-5H2,1H3
- Substance type: Organic
- Physical state: Liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material : (3-aminopropyl)(methyl)amine; 3-Amino-1-methylaminopropane
- Molecular formula : C4H12N2
- Molecular weight : 88.1528 g/mol
- Smiles notation : N(CCCN)C
- InChl : 1S/C4H12N2/c1-6-4-2-3-5/h6H,2-5H2,1H3
- Substance type: Organic
- Physical state: Liquid
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: Salmonella typhimurium LT2 strains TA 1950 (hisG46, uvrB} ; TS24 (hisG46,recA); TA1537 (hisC3076, uvrB, $a); TA1538 (hisD3052, uvrB, $a}; TA1952(hisC3076, uvrB); and mutant hisG46
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- Not specified
- Vehicle / solvent:
- Not specified
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Not specified
- Rationale for test conditions:
- Not specified
- Evaluation criteria:
- Evaluation was done considering a dose dependent increase in the number of revertants/plate.
- Statistics:
- Not specified
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: Salmonella typhimurium LT2 strains TA 1950 (hisG46, uvrB} ; TS24 (hisG46,recA); TA1537 (hisC3076, uvrB, $a); TA1538 (hisD3052, uvrB, $a}; TA1952(hisC3076, uvrB); and mutant hisG46
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- The test substance result was considered to be negative when tested seprately.While oligoamine-nitrate reaction mixtures produce mutagenic effect in all strain tested for mutagenicity.
- Remarks on result:
- other: No mutagenic effect were observed.
Applicant's summary and conclusion
- Conclusions:
- N-Methyl-1,3-propanediamine (6291-84-5)was evaluated for its mutagenic potential inSalmonella typhimurium LT2 strains TA 1950 (hisG46, uvrB} ; TS24 (hisG46,recA); TA1537 (hisC3076, uvrB, $a); TA1538 (hisD3052, uvrB, $a}; TA1952(hisC3076, uvrB); and mutant hisG46. The test result was observed to be negative.
- Executive summary:
Genetic toxicity in vitro study for N-Methyl-1,3-propanediamine was assessed for its possible mutagenic potential. For this purpose Bacterial reverse mutation assay was performed in Salmonella typhimurium LT2 strains TA 1950 (hisG46, uvrB} ; TS24 (hisG46,recA); TA1537 (hisC3076, uvrB, $a); TA1538 (hisD3052, uvrB, $a}; TA1952(hisC3076, uvrB); and mutant hisG46. No mutagenic effect were observed .Therefore N-Methyl-1,3-propanediamine was considered to be non mutagenic. Hence the test substance cannot be classified as gene mutant in vitro.
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