Bis(2-chloroethyl) ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

The assay of acute vapor toxicity, and the grading and interpretation of results on 96 chemical compounds.

Author:

Carpenter CP, Smyth HF, Pozzani UC

Year:

1949

Bibliographic source:

J. Ind. Hyg. Toxicol. vol. 31 no. 6, pp. 343-346

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

Age of test animals not specified. Weight variation among test animals not specified. Acclimation period not specified. Unclear if negative control animals were used. No analytical check of the concentration of the prepared vapour.

GLP compliance:

no

Remarks:

pre-GLP

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2-chloroethyl) ether

EC Number:

203-870-1

EC Name:

Bis(2-chloroethyl) ether

Cas Number:

111-44-4

Molecular formula:

C4H8Cl2O

IUPAC Name:

1-chloro-2-(2-chloroethoxy)ethane

Test material form:

liquid

Specific details on test material used for the study:

TEST MATERIA
- Name (as cited): 2, 2' -dichloroethyl ether
- Purity: not specified

Test animals

Species:

rat

Strain:

Sherman

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Mellon institute in-house breeding
- Age at study initiation: not specified
- Weight at study initiation: 100-150 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

Not specified

No. of animals per sex per dose:

6

Control animals:

not specified

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

ca. 250 ppm

Based on:

test mat.

Remarks:

nominal concentration

Exp. duration:

4 h

Remarks on result:

other: equivalent to 1.464 mg/L

Mortality:

No detailed mortality data are reported

Clinical signs:

other: The data are not reported

Body weight:

The data are not reported

Gross pathology:

The data are not reported

Applicant's summary and conclusion

Interpretation of results:

Category 2 based on GHS criteria

Conclusions:

In the conditions of this test, exposure to vapour of Bis(2-chloroethyl) ether at 250 ppm led to the death of 2 to 4 animals (out of 6). The LC50 is thus approximatively 250 ppm or 1.464 mg/L. This data classifies the substance in the Acute Category II according to the CLP criteria.

Executive summary:

In this publication, 96 compounds were graded on the basis of the response of rats to a 4-hour vapor exposure followed by a 14-day observation period. The methodological aspects of the study are described in sufficient details and, despite detailed results are not reported, the data are judged reliable.

In the conditions of this test, exposure to vapour of Bis(2-chloroethyl) ether at 250 ppm led to the death of 2 to 4 animals (out of 6). No results regarding clinical signs or gross pathology are reported. Based on these observation, the LC50 in Sherman rats is thus approximatively 250 ppm or 1.464 mg/L Bis(2 -chloroethyl) ether. This data classifies the substance in the Acute Category II according to the CLP criteria.