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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List VI
Author:
Henry F. Smyth Jr., Charles P. Carpenter, Carrol S. Well, Urbano C. Pozzani & Jean A. Striegel
Year:
1962
Bibliographic source:
American Industrial Hygiene Association Journal, 1962, 23:2, 95-107

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
An eye irritation test was conducted to assess the extent of ocular injury caused by the test chemical 1-methylpiperazine (CAS No: 109-01-3) in rabbits.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-Methylpiperazine
Cas Number:
109-01-3
Molecular formula:
C5H12N2
IUPAC Name:
1-Methylpiperazine
Test material form:
liquid
Details on test material:
- Name of test material :1-Methylpiperazine
- Molecular formula :C5H12N2
- Molecular weight:100.1638 g/mole
- Substance type:Organic
- Smiles notation: N1(CCNCC1)C
- InChl: 1S/C5H12N2/c1-7-4-2-6-3-5-7/h6H,2-5H2,1H3
- Physical state: Liquid
Specific details on test material used for the study:
- Name of test material: 1-Methylpiperazine
- Molecular formula: C5H12N2
- Molecular weight: 100.1638 g/mole
- Substance type: Organic
- Smiles notation: N1(CCNCC1)C
- InChl: 1S/C5H12N2/c1-7-4-2-6-3-5-7/h6H,2-5H2,1H3
- Physical state: Liquid

Test animals / tissue source

Species:
rabbit
Strain:
other: Albino
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
not specified
Controls:
yes
Amount / concentration applied:
Not reported
Duration of treatment / exposure:
No data
Observation period (in vivo):
No data
Duration of post- treatment incubation (in vitro):
No data
Details on study design:
TEST SITE
Area of exposure: eye
% coverage: No data
Type of wrap if used: no wrap used

REMOVAL OF TEST SUBSTANCE
Washing (if done): no data
Time after start of exposure: no data

SCORING SYSTEM: Eye injury in rabbits is recorded in a 10- grade ordinal series and is based upon the
degree of corneal necrosis that results from instillation of various volumes and concentrations of chemical Grade 1 indicates at most a very small area of necrosis resulting from 0.5 ml of undiluted chemical in the eye. Grade 5 indicates a so-called severe burn from 0.005 ml, and Grade 10 indicates a severe burn from 0.5 ml of a 1% solution in water or propylene glycol.

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Severe irritation was observed in treated animals.

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
Primary Eye Irritation score for 1-methylpiperazine (CAS No: 109-01-3) was Grade 8. Based on the grade, 1-methylpiperazine (CAS No: 109-01-3) was considered to be severely irritating to eyes.
Executive summary:

An eye irritation test was conducted to assess the extent of ocular injury caused by the test chemical 1-methylpiperazine (CAS No: 109-01-3) in rabbits.

 

In this test,eye injury in rabbits was recorded in a 10- grade ordinal series and was based upon the degree of corneal necrosis that results from instillation of various volumes and concentrations of chemical1-methyl piperazine.

 

Grade 1 indicated at most a very small area of necrosis resulting from 0.5 ml of undiluted chemical in the eye. Grade 5 indicated a so-called severe burn from 0.005 ml, and Grade 10 indicated a severe burn from 0.5 ml of a 1% solution in water or propylene glycol.

 

Primary Eye Irritation score for1-methyl piperazinewas observed to be Grade 8. Thusbased on the grade, 1-methylpiperazine (CAS No: 109-01-3)was considered to be severely irritating to eyes.